Annexin-V: Difference between revisions
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Latest revision as of 06:29, 5 May 2009
Purpose
- Stains cells in early stage of apoptosis
- Binds selectively to phosphatidylserine, which is flipped to the outside of cell membrane early in apoptosis
Procurement
- Standard companies
Use
- Conjugated to fluorochrome of your choice (FITC, PE, Biotin, etc.)
- Used in conjunction with vital dye (e.g. PI, 7-AAD) to distinguish dead cells from apoptotic cells
- Annexin-V +/7-AAD +: Dead
- Annexin-V +/7-AAD -: Apoptotic
- BD Biosciences protocol
Safety
- No significant considerations (BD Biosciences MSDS Sheet)
