Annexin-V

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* Conjugated to other fluorochromes (PE, FITC)
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==Purpose==
* Stains cells in '''early stage of apoptosis'''
* Stains cells in '''early stage of apoptosis'''
** Binds selectively to phosphatidylserine, which is flipped to the outside of cell membrane early in apoptosis
** Binds selectively to phosphatidylserine, which is flipped to the outside of cell membrane early in apoptosis
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* Must be used in conjunction with vital dye (e.g. [[Propidium iodide|PI]], [[7-AAD]]) to distinguish dead cells (Annexin-V +/7-AAD +) from apoptotic cells (Annexin-V +/7-AAD -)
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==Procurement==
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* Standard companies
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==Use==
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* Conjugated to fluorochrome of your choice (FITC, PE, Biotin, etc.)
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* Used in conjunction with vital dye (e.g. [[Propidium iodide|PI]], [[7-AAD]]) to distinguish dead cells from apoptotic cells
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** Annexin-V +/7-AAD +: Dead
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** Annexin-V +/7-AAD -: Apoptotic
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* [http://www.bdbiosciences.com/pharmingen/protocols/Annexin_V_Staining.shtml BD Biosciences protocol]
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<!-- *Include any relevant links to [[Protocols|OWW protocols]]
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*Include any relevant links to the manufacturer's protocols -->
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==Safety==
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* No significant considerations ([http://www.bdbiosciences.com/external_files/pm/doc/msds/live/web_enabled/556417-MSDS.00.pdf BD Biosciences MSDS Sheet])
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<!-- ==References==
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*Any papers or websites that are relevant to the use of this material
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==Contact==
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*Who has experience using this material?
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[[Category:Material]]

Revision as of 02:55, 8 July 2008

Contents

Purpose

  • Stains cells in early stage of apoptosis
    • Binds selectively to phosphatidylserine, which is flipped to the outside of cell membrane early in apoptosis

Procurement

  • Standard companies

Use

  • Conjugated to fluorochrome of your choice (FITC, PE, Biotin, etc.)
  • Used in conjunction with vital dye (e.g. PI, 7-AAD) to distinguish dead cells from apoptotic cells
    • Annexin-V +/7-AAD +: Dead
    • Annexin-V +/7-AAD -: Apoptotic
  • BD Biosciences protocol

Safety

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