Annealing primers

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Annealing primers can be used as a fast and cheap way to synthesize a short piece of DNA for which you do not have template DNA to PCR from.  See [[Synthetic Biology:BioBricks/Part fabrication|part fabrication]] for other ways to make a part (contains [[Synthetic Biology:BioBricks|BioBrick]] specific details).
Annealing primers can be used as a fast and cheap way to synthesize a short piece of DNA for which you do not have template DNA to PCR from.  See [[Synthetic Biology:BioBricks/Part fabrication|part fabrication]] for other ways to make a part (contains [[Synthetic Biology:BioBricks|BioBrick]] specific details).
#[[Annealing complementary primers]]<--For pieces of DNA shorter than the limit on primer length.
#[[Annealing complementary primers]]<--For pieces of DNA shorter than the limit on primer length.
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#[[Annealing and primer extension]]<--For pieces of DNA longer than the limit on primer lenght
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#[[Annealing and primer extension]]<--For pieces of DNA longer than the limit on primer length.

Revision as of 13:55, 26 May 2006

Annealing primers can be used as a fast and cheap way to synthesize a short piece of DNA for which you do not have template DNA to PCR from. See part fabrication for other ways to make a part (contains BioBrick specific details).

  1. Annealing complementary primers<--For pieces of DNA shorter than the limit on primer length.
  2. Annealing and primer extension<--For pieces of DNA longer than the limit on primer length.
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