Ampicillin - Revision history

Tk: /* Stability */

2008-02-10T04:46:17Z

Stability

←Older revision		Revision as of 04:46, 10 February 2008
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	==Stability==		==Stability==

-	Culture plates with ampicillin can be stored at 2-~~8 °C~~ for up to two weeks. (In our experience, plates are usable for 2-3 months when stored at 4°C and bagged to prevent evaporation. -[[User:Tk\|tk]]) Stock solutions may be stored at 2-~~8 °C~~ for up to 3 weeks. For long term storage (4-6 months), stock solutions should be stored at -~~20 °C. Dissolving ampicillin in 50% ethanol rather than water allows it to be stored in liquid form at -20 °C~~. At ~~37 ° C~~ in culture, ampicillin is stable up to 3 days. [http://www.sigmaaldrich.com/sigma/product%20information%20sheet/a0166pis.pdf Sigma reference]	+	Culture plates with ampicillin can be stored at 2-8°C for up to two weeks. (In our experience, plates are usable for 2-3 months when stored at 4°C and bagged to prevent evaporation. -[[User:Tk\|tk]]) Stock solutions may be stored at 2-8°C for up to 3 weeks. For long term storage (4-6 months), stock solutions should be stored at -20°C. At 37°C in culture, ampicillin is stable up to 3 days. [http://www.sigmaaldrich.com/sigma/product%20information%20sheet/a0166pis.pdf Sigma reference]

	==Usage Notes==		==Usage Notes==

Tk: /* Stock Solution */

2008-02-10T04:45:11Z

Stock Solution

←Older revision		Revision as of 04:45, 10 February 2008
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	==Stock Solution==		==Stock Solution==

-	Typical concentrations of ampicillin are 50 ug/ml for low copy plasmids and 100 ug/ml for high copy plasmids. Stock solutions are typically at 100 mg/ml, so that 1 ml of antibiotic can be added to 1 liter of broth or agar. Stock solutions made in 50% alcohol remain liquid at -~~20 C~~ and are easy to pipet. Cool agar to ~~55C~~ or below prior to adding antibiotic.	+	Typical concentrations of ampicillin are 50 μg/mL for low copy plasmids and 100 μg/mL for high copy plasmids. Stock solutions are typically at 100 mg/ml, so that 1 ml of antibiotic can be added to 1 liter of broth or agar. Stock solutions made in 50% alcohol remain liquid at -20°C and are easy to pipet. Cool agar to 55°C or below prior to adding antibiotic.

	Ampicillin available from Sigma A-9518 (Ampicillin sodium salt), FW 371.39. To make 100ml of 100 mg/ml stock solution, dissolve 10 g of ampicillin in 50 ml of water and 50 ml of 100% ethanol.		Ampicillin available from Sigma A-9518 (Ampicillin sodium salt), FW 371.39. To make 100ml of 100 mg/ml stock solution, dissolve 10 g of ampicillin in 50 ml of water and 50 ml of 100% ethanol.

Tk: /* Stability */

2008-02-10T04:41:56Z

Stability

←Older revision		Revision as of 04:41, 10 February 2008
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	==Stability==		==Stability==

-	Culture plates with ampicillin can be stored at 2-8 °C for up to two weeks. Stock solutions may be stored at 2-8 °C for up to 3 weeks. For long term storage (4-6 months), stock solutions should be stored at -20 °C. At 37 ° C in culture, ampicillin is stable up to 3 days. [http://www.sigmaaldrich.com/sigma/product%20information%20sheet/a0166pis.pdf Sigma reference]	+	Culture plates with ampicillin can be stored at 2-8 °C for up to two weeks. (In our experience, plates are usable for 2-3 months when stored at 4°C and bagged to prevent evaporation. -[[User:Tk\|tk]]) Stock solutions may be stored at 2-8 °C for up to 3 weeks. For long term storage (4-6 months), stock solutions should be stored at -20 °C. Dissolving ampicillin in 50% ethanol rather than water allows it to be stored in liquid form at -20 °C. At 37 ° C in culture, ampicillin is stable up to 3 days. [http://www.sigmaaldrich.com/sigma/product%20information%20sheet/a0166pis.pdf Sigma reference]

	==Usage Notes==		==Usage Notes==

Reshma P. Shetty at 03:51, 21 December 2007

2007-12-21T03:51:57Z

←Older revision		Revision as of 03:51, 21 December 2007
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	==References==		==References==
-	~~Molecular Cloning: A Laboratory Manual, Vol 1.~~	+	<biblio>
		+	#Anderson-Lancet-1965 pmid=14238093
		+	#Datta-BiochemJ-1966 pmid=5328167
		+	#Evans-JBacteriol-1968 pmid=4971890
		+	#MolecularCloning isbn=0-87969-577-3
		+	</biblio>

	[[Category:Material]] [[Category:Antibiotic]]		[[Category:Material]] [[Category:Antibiotic]]

ClarkeS: typo

2007-09-13T16:19:12Z

typo

←Older revision		Revision as of 16:19, 13 September 2007
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	==Usage Notes==		==Usage Notes==
-	A 1990 [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2199796 paper] by [http://www.biology.bnl.gov/cellbio/studier.html Bill Studier] discusses how the secreted [[β-lactamase]] can quickly consume all the ampicillin in a culture (even at 20μg/ml Amp). A stationary culture of ampicillin resistant cells can have such a concentration of [[β-lactamase]] that even a 1/200 to 1/1000 dilution will still contain enough [[β-lactamase]] to consume all the fresh ampicillin before all the non-resistant cells from the stationary phase culture have been killed. The authors ~~reccommend~~ not allowing cultures to reach stationary phase if you need a high proportion of cells to contain your plasmid.	+	A 1990 [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2199796 paper] by [http://www.biology.bnl.gov/cellbio/studier.html Bill Studier] discusses how the secreted [[β-lactamase]] can quickly consume all the ampicillin in a culture (even at 20μg/ml Amp). A stationary culture of ampicillin resistant cells can have such a concentration of [[β-lactamase]] that even a 1/200 to 1/1000 dilution will still contain enough [[β-lactamase]] to consume all the fresh ampicillin before all the non-resistant cells from the stationary phase culture have been killed. The authors recommend not allowing cultures to reach stationary phase if you need a high proportion of cells to contain your plasmid.
	*[[Barry Canton]] & [[Matt Gethers]] have seen this occur when using the BioBrick plasmid, pSB1A3 expressing a high level of mCherry.		*[[Barry Canton]] & [[Matt Gethers]] have seen this occur when using the BioBrick plasmid, pSB1A3 expressing a high level of mCherry.

Reshma P. Shetty at 17:44, 26 July 2007

2007-07-26T17:44:59Z

←Older revision		Revision as of 17:44, 26 July 2007
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	==References==		==References==
	Molecular Cloning: A Laboratory Manual, Vol 1.		Molecular Cloning: A Laboratory Manual, Vol 1.
		+
		+	[[Category:Material]] [[Category:Antibiotic]]

Austin J. Che at 19:25, 27 April 2007

2007-04-27T19:25:58Z

←Older revision		Revision as of 19:25, 27 April 2007
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	Culture plates with ampicillin can be stored at 2-8 °C for up to two weeks. Stock solutions may be stored at 2-8 °C for up to 3 weeks. For long term storage (4-6 months), stock solutions should be stored at -20 °C. At 37 ° C in culture, ampicillin is stable up to 3 days. [http://www.sigmaaldrich.com/sigma/product%20information%20sheet/a0166pis.pdf Sigma reference]		Culture plates with ampicillin can be stored at 2-8 °C for up to two weeks. Stock solutions may be stored at 2-8 °C for up to 3 weeks. For long term storage (4-6 months), stock solutions should be stored at -20 °C. At 37 ° C in culture, ampicillin is stable up to 3 days. [http://www.sigmaaldrich.com/sigma/product%20information%20sheet/a0166pis.pdf Sigma reference]
-
-	~~==Plate color code==~~
-	~~Orange stripe~~

	==Usage Notes==		==Usage Notes==

Josh K. Michener at 18:01, 7 August 2006

2006-08-07T18:01:35Z

←Older revision		Revision as of 18:01, 7 August 2006
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	Ampicillin available from Sigma A-9518 (Ampicillin sodium salt), FW 371.39. To make 100ml of 100 mg/ml stock solution, dissolve 10 g of ampicillin in 50 ml of water and 50 ml of 100% ethanol.		Ampicillin available from Sigma A-9518 (Ampicillin sodium salt), FW 371.39. To make 100ml of 100 mg/ml stock solution, dissolve 10 g of ampicillin in 50 ml of water and 50 ml of 100% ethanol.
		+
		+	==Stability==
		+
		+	Culture plates with ampicillin can be stored at 2-8 °C for up to two weeks. Stock solutions may be stored at 2-8 °C for up to 3 weeks. For long term storage (4-6 months), stock solutions should be stored at -20 °C. At 37 ° C in culture, ampicillin is stable up to 3 days. [http://www.sigmaaldrich.com/sigma/product%20information%20sheet/a0166pis.pdf Sigma reference]

	==Plate color code==		==Plate color code==

Barry Canton: /* Usage Notes */

2006-08-02T14:25:51Z

Usage Notes

←Older revision		Revision as of 14:25, 2 August 2006
Line 18:		Line 18:

	==Usage Notes==		==Usage Notes==
-	A 1990 [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2199796 paper] by [http://www.biology.bnl.gov/cellbio/studier.html Bill Studier] discusses how the secreted [[β-lactamase]] can quickly consume all the ampicillin in a culture (even at 20μg/ml Amp). A stationary culture of ampicillin resistant cells can have such a concentration of [[β-lactamase]] that even a 1/200 to 1/1000 dilution will still contain enough [[β-lactamase]] to consume all the fresh ampicillin before all the non-resistant cells from the stationary phase culture have been killed.	+	A 1990 [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=pubmed&dopt=Abstract&list_uids=2199796 paper] by [http://www.biology.bnl.gov/cellbio/studier.html Bill Studier] discusses how the secreted [[β-lactamase]] can quickly consume all the ampicillin in a culture (even at 20μg/ml Amp). A stationary culture of ampicillin resistant cells can have such a concentration of [[β-lactamase]] that even a 1/200 to 1/1000 dilution will still contain enough [[β-lactamase]] to consume all the fresh ampicillin before all the non-resistant cells from the stationary phase culture have been killed. The authors reccommend not allowing cultures to reach stationary phase if you need a high proportion of cells to contain your plasmid.
		+	*[[Barry Canton]] & [[Matt Gethers]] have seen this occur when using the BioBrick plasmid, pSB1A3 expressing a high level of mCherry.

-	~~The authors reccommend not allowing cultures to reach stationary phase if you need a high proportion of cells to contain your plasmid.~~

	[[Carbenicillin]] is much more resistant and would be preferred except for cost. Mixtures of ampicillin and carbenicillin are often used.		[[Carbenicillin]] is much more resistant and would be preferred except for cost. Mixtures of ampicillin and carbenicillin are often used.

Alexis: /* Mode of Action */

2006-06-13T20:58:13Z

Mode of Action

←Older revision		Revision as of 20:58, 13 June 2006
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	==Mode of Action==		==Mode of Action==
-	Inhibits the formation of cross-links in the peptidoglycan layer (which ~~prvides~~ rigidity to the cell wall). Most effective against cells in log phase growth (since this is when new cross-links are being formed), and has little effect on cells in stationary phase.	+	Inhibits the formation of cross-links in the peptidoglycan layer (which provides rigidity to the cell wall). Most effective against cells in log phase growth (since this is when new cross-links are being formed), and has little effect on cells in stationary phase.

	==Mechanism of Resistance==		==Mechanism of Resistance==