Ampicillin: Difference between revisions
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==Mechanism of Resistance== | ==Mechanism of Resistance== | ||
Expression of beta-lactamase neutralizes ampicillin. When this enzyme | Expression of [[β-lactamase]] neutralizes ampicillin. When this enzyme is expressed on a high-copy number plasmid there is significant diffusion into the extracellular medium. As a result non-resistant satellite colonies may form around larger resistant colonies. | ||
(would be nice to have a picture of this here) | |||
==References== | ==References== | ||
Molecular Cloning: A Laboratory Manual, Vol 1. | Molecular Cloning: A Laboratory Manual, Vol 1. | ||
Revision as of 11:08, 11 May 2005
Mode of Action
Inhibits the formation of cross-links in the peptidoglycan layer (which prvides rigidity to the cell wall). Most effctive against cells in log phase growth (since this is when new cross-links are being formed), and has little effect on cells in stationary phase.
Mechanism of Resistance
Expression of β-lactamase neutralizes ampicillin. When this enzyme is expressed on a high-copy number plasmid there is significant diffusion into the extracellular medium. As a result non-resistant satellite colonies may form around larger resistant colonies.
(would be nice to have a picture of this here)
References
Molecular Cloning: A Laboratory Manual, Vol 1.
