Amine coupling method in SPR: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
No edit summary |
||
Line 1: | Line 1: | ||
{|border=1 | {|border=1 | ||
|protein||conditions||citation | |protein||conditions||immobilization level||citation | ||
|- | |- | ||
|HSA (Human Serum Albumin) | |HSA (Human Serum Albumin) | ||
Line 8: | Line 8: | ||
(4) 50 mM NaOH, 3x30s pulse wash (remove uncoupled protein) | (4) 50 mM NaOH, 3x30s pulse wash (remove uncoupled protein) | ||
|<cite>karlsson1</cite> | |<cite>karlsson1</cite> | ||
|9,500-11,000 | |||
|} | |} | ||
Revision as of 14:24, 7 April 2008
protein | conditions | immobilization level | citation |
HSA (Human Serum Albumin) | (1) 0.2M EDC + 50 mM NHS in 10 mM NaOAc pH 5.2, 7 min (2) HSA, 15 μg/mL same buffer 7 min |
[1] | 9,500-11,000 |
- Frostell-Karlsson A, Remaeus A, Roos H, Andersson K, Borg P, Hämäläinen M, and Karlsson R. Biosensor analysis of the interaction between immobilized human serum albumin and drug compounds for prediction of human serum albumin binding levels. J Med Chem. 2000 May 18;43(10):1986-92. DOI:10.1021/jm991174y |