Altman:Protocols/TC Protocols/Thawing ARPE-19: Difference between revisions

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(New page: {{Template:Altman}} <div style="padding: 10px; width: 700px; border: 5px solid #B22222;"> ==Splitting ARPE-19 cells== Before starting: * Warm up Media+Serum in 37°C water-bath * Place ...)
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Revision as of 19:01, 4 September 2013


Department of Physics, Willamette University

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Splitting ARPE-19 cells

Before starting:

  • Warm up Media+Serum in 37°C water-bath
  • Place a beaker for disposing of waste in the hood
  • Place a T-25 Flask in the hood
  • Place 9 mL of warmed Media+Serum into a 15 mL conical


1. Thaw aliquot of cells in 37°C water-bath, making sure to avoid submerging the cap and o-ring; this should take about 2 minutes

2. Move cells into the 9 mL of media

3. Spin cells at 500 rpm for 3 minutes

4. Remove supernatant

5. Re-suspend the pellet in 5 mL of media

6. Place the cells into a T-25 flask

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