Altman:Protocols/TC Protocols/SF9 conditions

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(New page: {{Template:Altman}} <div style="padding: 10px; width: 700px; border: 5px solid #B22222;"> ==Maintaining SF9 cells== * Cells are maintained in 125 mL disposable sterile Erlenmeyer flasks...)
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==Maintaining SF9 cells==
==Maintaining SF9 cells==
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* Cells are maintained in 125 mL disposable sterile Erlenmeyer flasks in SF-900 II SFM (Gibco) + 100x Pen-Strep-Glutamine (Life Technologies) at 28 C
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* Cells are maintained in 125 mL disposable sterile Erlenmeyer flasks in SF-900 II SFM (Gibco) + 100x Pen-Strep-Glutamine (Life Technologies) at 28 C shaking at 150 rpm
* Once cell culture density has reached >2*10^6 viable cells/mL, the cells are split to a density of 3-5 viable cells/mL
* Once cell culture density has reached >2*10^6 viable cells/mL, the cells are split to a density of 3-5 viable cells/mL

Revision as of 20:42, 4 September 2013


Department of Physics, Willamette University

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Maintaining SF9 cells

  • Cells are maintained in 125 mL disposable sterile Erlenmeyer flasks in SF-900 II SFM (Gibco) + 100x Pen-Strep-Glutamine (Life Technologies) at 28 C shaking at 150 rpm
  • Once cell culture density has reached >2*10^6 viable cells/mL, the cells are split to a density of 3-5 viable cells/mL
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