Altman:Protocols/TC Protocols/SF9 conditions: Difference between revisions
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David Altman (talk | contribs) (New page: {{Template:Altman}} <div style="padding: 10px; width: 700px; border: 5px solid #B22222;"> ==Maintaining SF9 cells== * Cells are maintained in 125 mL disposable sterile Erlenmeyer flasks...) |
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Revision as of 12:26, 24 July 2013
Maintaining SF9 cells
- Cells are maintained in 125 mL disposable sterile Erlenmeyer flasks in SF-900 II SFM (Gibco) + 100x Pen-Strep-Glutamine (Life Technologies) at 28 C
- Once cell culture density has reached >2*10^6 viable cells/mL, the cells are split to a density of 3-5 viable cells/mL