Altman:Protocols/TC Protocols/SF9 conditions: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
David Altman (talk | contribs) No edit summary |
David Altman (talk | contribs) |
||
| Line 6: | Line 6: | ||
* Cells are maintained in 125 mL disposable sterile Erlenmeyer flasks in SF-900 II SFM (Gibco) + 100x Pen-Strep-Glutamine (Life Technologies) at 28 C shaking at 150 rpm | * Cells are maintained in 125 mL disposable sterile Erlenmeyer flasks in SF-900 II SFM (Gibco) + 100x Pen-Strep-Glutamine (Life Technologies) at 28 C shaking at 150 rpm | ||
* Once cell culture density has reached >2*10^6 viable cells/mL, the cells are split to a density of 3-5 viable cells/mL | * Once cell culture density has reached >2*10^6 viable cells/mL, the cells are split to a density of 3-5*10^5 viable cells/mL | ||
Latest revision as of 08:47, 8 September 2014
Department of Physics, Willamette University
Maintaining SF9 cells
- Cells are maintained in 125 mL disposable sterile Erlenmeyer flasks in SF-900 II SFM (Gibco) + 100x Pen-Strep-Glutamine (Life Technologies) at 28 C shaking at 150 rpm
- Once cell culture density has reached >2*10^6 viable cells/mL, the cells are split to a density of 3-5*10^5 viable cells/mL
