Altman:Protocols/Single Molecule Assays/gliding filament

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(New page: {{Template:Altman}} <div style="padding: 10px; width: 700px; border: 5px solid #B22222;"> ==Making Nitrocellulose Coverslips== // Typical flow-through volumes are 10-15 μL // 1. Make ...)
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Revision as of 15:45, 15 June 2013


Department of Physics, Willamette University

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Making Nitrocellulose Coverslips

// Typical flow-through volumes are 10-15 μL //

1. Make a flow cell with a nitrocellulose-coated coverslip 2. Flow in α-GFP/10 3. Incubate 2 minutes 4. Flow in ABSA 5. Incubate 2 minutes 6. Flow in motor dilution 7. Incubate 2 minutes 8. Flow in ABSA 9. Flow in Actin dilution 10. Incubate 2 minutes 11. Flow in GO-Juice


Recipes

50 uL Buffer #1
50x GOC stock 2 μL
100x CPK stock 1 μL
100x ATP stock 1 μL
AB 46 μL


50 μL Buffer #2
50x Glu/B stock 2 μL
100x PCr stock 1 μL
AB 47 μL
20 μL GO Juice
Buffer #1 10 μL
Buffer #2 10 μL


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