Altman:Protocols/Preparing Reagents/Making nitrocellulose coverslips

Department of Physics, Willamette University

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Making Nitrocellulose Coverslips

1. Make bead/Triton mixture: 1 μL beads + 199 μL 0.2% Triton X-100

2. Spread 7.5 μL on each slide

3. Allow coverslips to dry

4. Dip coverslips in 0.1% collodion in amyl acetate

Recipes

	2.5 mL 0.2% Triton
25% Triton X-100	20 μL
sterile water	2.5 mL

	20 mL 0.1% Collodion
2% collodion in amyl acetate	1 mL
Amyl acetate	19 mL