Altman:Protocols/Preparing Reagents/Making nitrocellulose coverslips

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Current revision (21:11, 18 November 2012) (view source)
 
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1. Make bead/Triton mixture: 1 μL beads + 199 μL 0.2% Triton X-100
1. Make bead/Triton mixture: 1 μL beads + 199 μL 0.2% Triton X-100
 +
2. Spread 7.5 μL on each slide
2. Spread 7.5 μL on each slide
 +
3. Allow coverslips to dry
3. Allow coverslips to dry
 +
4. Dip coverslips in 0.1% collodion in amyl acetate
4. Dip coverslips in 0.1% collodion in amyl acetate
Recipes
Recipes
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<table id="Table 1" border="1" cellpadding="3" cellspacing="0">

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Department of Physics, Willamette University

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Making Nitrocellulose Coverslips

1. Make bead/Triton mixture: 1 μL beads + 199 μL 0.2% Triton X-100

2. Spread 7.5 μL on each slide

3. Allow coverslips to dry

4. Dip coverslips in 0.1% collodion in amyl acetate


Recipes

2.5 mL 0.2% Triton
25% Triton X-100 20 μL
sterile water 2.5 mL


20 mL 0.1% Collodion
2% collodion in amyl acetate 1 mL
Amyl acetate 19 mL
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