Altman:Protocols/Preparing Reagents/Making PCR: Difference between revisions
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* Creatine Phosphate Disodium Salt – Calbiochem, Cat #: 2380, 5 gm or 25 gm, store at -20°C, MW is 255.1 | * Creatine Phosphate Disodium Salt – Calbiochem, Cat #: 2380, 5 gm or 25 gm, store at -20°C, MW is 255.1 | ||
* Final stock (100x) should be 100 mM stored in 1x AB | * Final stock (100x) should be 100 mM PCr stored in 1x AB | ||
Latest revision as of 18:05, 18 November 2012
Department of Physics, Willamette University
Making Creatine Phosphate Disodium Salt (PCr)
- Creatine Phosphate Disodium Salt – Calbiochem, Cat #: 2380, 5 gm or 25 gm, store at -20°C, MW is 255.1
- Final stock (100x) should be 100 mM PCr stored in 1x AB
| 100 mM PCr | |
|---|---|
| 1x AB | 3.920 mL |
| PCr | 0.1 g |
- Aliquot 50 μL stocks in the cold-room
- Store at -20°C
