Altman:Protocols/Preparing Reagents/Making ATP stock solution

From OpenWetWare

< Altman:Protocols | Preparing Reagents(Difference between revisions)
Jump to: navigation, search
(New page: {{Template:Altman}} <div style="padding: 10px; width: 700px; border: 5px solid #B22222;"> ==Making Nitrocellulose Coverslips== 1. Add 551 mg of Adenosine 5′-triphosphate disodium sal...)
Current revision (12:22, 12 March 2013) (view source)
 
Line 3: Line 3:
<div style="padding: 10px; width: 700px; border: 5px solid #B22222;">
<div style="padding: 10px; width: 700px; border: 5px solid #B22222;">
-
==Making Nitrocellulose Coverslips==
+
==Making 0.1 M ATP stock solution==
-
1. Add 551 mg of Adenosine 5′-triphosphate disodium salt hydrate (A26209 Aldrich)to 8 mL of diwater
+
1. Add 551 mg of Adenosine 5′-triphosphate disodium salt hydrate (A26209 Aldrich, MW 551.14) to 8 mL of diwater
2. Adjust to pH 7.0 using 1 M  KOH solution
2. Adjust to pH 7.0 using 1 M  KOH solution

Current revision


Department of Physics, Willamette University

'


Home        Contact        Lab Members        Publications        Research        Protocols        Pictures       


Making 0.1 M ATP stock solution

1. Add 551 mg of Adenosine 5′-triphosphate disodium salt hydrate (A26209 Aldrich, MW 551.14) to 8 mL of diwater

2. Adjust to pH 7.0 using 1 M KOH solution

3. Raise to 10 mL with diwater

4. Aliquot (500 uL & 50 uL) and store in -20
Personal tools