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Hints from a technician in the Endy lab: Basic plasticware (gloves, pipette tips, eppendorf tubes) we get from [[http://www.bioexpress.com ISC Bioexpress]].  They are by far the cheapest but to get those prices you have to deal with the company rep. who issues a price quote.  USA Scientific (a sister company of Eppendorf) is also a good source for plasticware and racks, etc and they issue a blank quote for all of MIT, so you just need to be in touch with the rep to get that quote number but it is only good for certain items.  A lot of people simply use the VWR stockroom in [[http://whereis.mit.edu/map-jpg?mapterms=56&mapsearch=go Building 56]] and just go and buy what they need as they need it.  You can also just start signing up for accounts with different companies online and start requesting their catalogs so you can get a feel for what they offer. With most companies you can also use Purchase Orders if you prefer or order through webpages with a credit card.
[[Alm:Internal]]


We should put through the application for an MIT Visa so we will have a more convenient way of spending from our accounts for things we can't get from SAPWeb or are cheaper somewhere else.
Hints from a technician in the Endy lab: Basic plasticware (gloves, pipette tips, eppendorf tubes) we get from [[http://www.bioexpress.com ISC Bioexpress]].  They are by far the cheapest but to get those prices you have to deal with the company rep. who issues a price quote.  USA Scientific (a sister company of Eppendorf) is also a good source for tubes, tips, and racks, and the lower prices are available through ecat3 on MIT's ecat.  A lot of people simply use the VWR stockroom in [[http://whereis.mit.edu/map-jpg?mapterms=56&mapsearch=go Building 56]] and just go and buy what they need as they need it.  You can also just start signing up for accounts with different companies online and start requesting their catalogs so you can get a feel for what they offer. With most companies you can also use Purchase Orders if you prefer or order through webpages with a credit card.  Eric has an MIT credit card.


I will sign up for catalogs with various companies and be the point person to deal with sales reps.<br>  Hopefully we can save some money up front this way. There are deals we can get as a lab just starting up.
[[User:ClarkeS|Sean]] 18:18, 29 June 2006 (EDT)


==Often-ordered product numbers==
S41873 Butane burner canisters (Replacement Fuel For S41872) (Fisher) ($7.85ea + 21.50 hazmat charge) Wall Lenk Vendor No.:65F
21008-959 Eppendorf Safe-Lock 1.5 mL Tubes Natural 022363204 Pack of 500 $34.27
9187-1000 Tough Tags for 1.5ml tubes 1.2x0.5" white (USA)
9185-0500 Tough Spots for 1.5ml tubes 0.5" diam. white (USA)
2344-1000 Tubular 4-way tube racks in set of 4 + tray (USA)
0497735 4 chisel tip dry erase markers (OD)
0254452 Uniball refills black  (OD)
32916-536 Large Evolution One gloves, case 500 (VWR)
32916-500 M Diamond Grip, CS 10 boxes (VWR)
60819-761 14ml Falcon (352059) dual-position snap-cap polypropylene culture tubes, case 500 (VWR)
25384-094 case of 600 (25 sleeves of 24) stackable for automation 100x15mm Petri plates  (VWR)
73520-774 Nunc large square plates DISH BIOASSAY LO PROFILE case 20 (VWR)
37000-700 galaxy micro centrifuge for spinning down tubes and strips $270 includes rotors/adaptors (VWR)
2004-00004 Silicone closures for flasks, 10 (Bellco Glass, Inc)
14220-010 Autoclave bags, clear with biohazard symbol (available without for $225.83), 12"x24" (slightly bigger?), 1.5 mil polypropylene Case of 1000 $243.64
95042-554 Autoclave bags, clear, 12"x24" 2mil thick polypropylene PK100 $33.05
WLS2493-A PK100 $19.95 VWR BAG AUTOCLAVE 12X24INPK100 Supplier: Tufpak (haven't tried these but they're cheaper)
==Vendor numbers==
*Fisher 142706


==Miscellaneous==
==Miscellaneous==


*Desk chairs: Office Depot (may be cheaper to go through regular site than the SAPwebpage)
*Desk chairs: Office Depot (may be cheaper to go through regular site than the SAPwebpage)
*Lab stools (look on ebay)
** Herman Miller through procurement office NE49 600 Tech Square 4th floor
 
*Lab stools
**VWR BioFit High Bench height (lab benches require chairs to go up to 29 inches)
**VWR Urethane lab stool
 
*Telephone [[http://web.mit.edu/ist/tel/csr-services.html MIT's phone service page]]
**All requests for new telephone service or changes in existing service should go through your department's Administrative Officer (AO).  Email customer service at telecom-csr@mit.edu or call 617-253-3670 for April-White Frager (Civil's rep).


==Lab-wide Equipment==
==Lab-wide Equipment==
 
*PCR machine (get loaners from companies, MJ great but only available used, Eppendorf used to be hard to use)
*Streamline Vertical Laminar FLow Cabinet SCV4A2 2007 20789
*Heat Block
*Eppendorf PCR machine
*Heat Blocks (dry baths)
*Stir Plate / Hot Plate
*Stir Plate / Hot Plate
*Waterbath (s)
*Waterbath (s)
*Incubators
*Incubators
**[[http://www.torreypinesscientific.com/ Torrey Pines]] brand, models IN35 and IN45: 4C-70C, 27L or 55L, see [[http://www.labx.com/v2/adsearch/detail3.cfm?adnumb=293161 description]]
**[[http://www.torreypinesscientific.com/ Torrey Pines]] brand, models IN35 and IN45: 4C-70C, 27L or 55L, see [[http://www.labx.com/v2/adsearch/detail3.cfm?adnumb=293161 description]]
*-20C Freezer (VWR or used)
*-20C Freezer (double-size, used from Broad, Fisher Sci model)
*4C Refrigerator (glass front?) (VWR or used)
*4C Refrigerator (glass front?) (VWR or used)
*Centrifuge for culture tubes (swinging bucket rotor?)
*Benchtop Centrifuge for culture tubes
*Table-top microcentrifuge (one refrigerated and one not, Eppendorf?)
*Microcentrifuge, Epp unrefrigerated
**[[http://www.labx.com/v2/adsearch/detail3.cfm?adnumb=293171 refurb Beckman Microfuge R]] from pegasusscientific.com
*Nanodrop
*Nanodrop
*Microbalance (enclosed, for up to __ g)
*Balance <400g
*Test tube roller
*Test tube roller
*Pressure cooker/hot plate for low cost autoclaving or small autoclave on wheels like on 4th floor
*Pressure cooker/hot plate for low cost autoclaving or small autoclave on wheels like on 4th floor
*Microbalance (enclosed, for up to __ g)
** NorthShore Care Supply: FREE GROUND SHIPPING on our entire line of Mettler Toledo Lab Balances. Coupon Code H25 should be used at online checkout or over the phone. [[http://www.northshorecare.com/mettler-lab-balances.html web page]]
*Balance (capacity?)


==Bench Top Equipment==  
==Bench Top Equipment==  
Mostly purchase from VWR
   
   
*Mini spin
*Mini spin, VWR
*2 Bunsen burners (I wouldn't recommend fireboys, they are nice and all, but expensive and are finicky)
*Burners
*Gas canisters (supplier?)
*Gas canisters
*2 Vortexers
*Butane torch and lighters
*2 Pipette sets (2.5, 20, 200 and 100 ul) (Gilson Pipetman, Eppendorf brands, find out what tips work, get samples)
*Vortexer
*Pipette sets (2.5, 20, 100?, 200 and 1000 ul) find out what tips work, get samples)
*Repeating pipet
*Pipet man (motorized pipetter)
*Pipet man (motorized pipetter)
*5? Thermometers
*Thermometers (glass for liquids and infra-red for surface measurements)
*3 Timers
*Timer


==Disposables/Plasticware==  
==Disposables/Plasticware==  
Line 50: Line 92:


*Pipette tips (with/without filter)
*Pipette tips (with/without filter)
*Gloves: Sean (S in latex, M in nitrile)
*Gloves latex, nitrile
*PCR tubes/plates
*PCR tubes/plates
*Eppendorf Tubes
*Eppendorf Tubes
*Conical Tubes (Falcon)
*15 ml Conical Tubes
*50 ml conical tubes
*14 ml culture tubes with two-position caps
*VWR brand lab markers
*VWR brand lab markers
*Lab Tape (Tape holder)
*Lab Tape (Tape holder)
Line 65: Line 109:
*Petri Plates (BD/Falcon?)
*Petri Plates (BD/Falcon?)
*Pasteur Pipettes (and bulbs)
*Pasteur Pipettes (and bulbs)
 
==Glassware/Pyrex==
==Glassware/Pyrex==


Line 74: Line 118:
*Media Bottles (we order from 50 ml up to 1 L)
*Media Bottles (we order from 50 ml up to 1 L)
*Beakers (maybe?)
*Beakers (maybe?)
*Test tubes
 
==Other items==
==Other items==
   
   
*Ice buckets
*Safety Glasses $7 at VWR
*Lab coats
*Test Tubes
*Test Tubes
*Glass Pipettes or Disposable Plastic Pipettes
*plastic pipettes
*Plastic Bins (greatly help with organization)
*Plastic Bins (greatly help with organization)
*Qiagen Kits
 
 
 
==Chemicals==  
==Chemicals==  


Line 90: Line 137:
*Antibiotics
*Antibiotics
*Protoplast fusion ingredients: EDTA, sodium maleate, etc.
*Protoplast fusion ingredients: EDTA, sodium maleate, etc.
*EtBr or SYBR Green/Gold
*EtBr or SYBR Green/Gold/Safe
 
==Enzymes==
==Enzymes==
   
   
Line 97: Line 144:
*PCR reagents: DNAPols, dNTPs
*PCR reagents: DNAPols, dNTPs
*Ligase
*Ligase
*Qiagen kits


==Shared with other labs or borrowed to begin with==
==Shared with other labs or borrowed to begin with==


*-80C Freezer (Chisholm)
*-80C Freezer (Chisholm)
*large centrifuge
*gel reader box and computer, UV gel viewer? (Polz)
*gel reader box and computer, UV table? (Polz) (ask Sri what to get, not VWR)
*MilliQ water (Polz/Chisholm/Delong)
*MilliQ water (Polz)
*Other spectrophotometer (or portable A600 spec ~$700)
*Other spectrophotometer (or portable A600 spec ~$700)


==Suppliers and Specialties==
==Suppliers==
 
===General===


* [[http://web.mit.edu/ecat/vwr/ VWR stockroom]] at MIT : most equipment, chemicals, convenient but not necessarily cheapest, can pick up in 56-070 or deliver?
* [http://web.mit.edu/ecat/vwr/ VWR stockroom] at MIT : most equipment, chemicals, convenient but not necessarily cheapest, can pick up some items in 56-070 or deliver next-day, carry Owl gel equipment
**ordered catalog [[User:ClarkeS|Sean]] 16:27, 30 June 2006 (EDT)
**ordered catalog [[User:ClarkeS|Sean]] 16:27, 30 June 2006 (EDT)


* [[http://www.usascientific.com/ USA Scientific]] (like Eppendorf just cheaper) : plastic racks,  ToughSpot labels, plasticware  
* [http://www.usascientific.com/ USA Scientific] Now in Ecat:Sciquest (like Eppendorf just cheaper) : plastic racks,  ToughSpot labels, plasticware  
**registered [[User:ClarkeS|Sean]]
**registered [[User:ClarkeS|Sean]]


* [[http://www.bioexpress.com ISC BioExpress]] : pipet tips, gloves, plasticware  
* [http://www.bioexpress.com ISC BioExpress] : pipet tips, gloves, plasticware  
**registered and account approved [[User:ClarkeS|Sean]] 17:02, 30 June 2006 (EDT)
**registered and account approved [[User:ClarkeS|Sean]] 17:02, 30 June 2006 (EDT)


* [[http://www.sigma.com Sigma]] : chemicals as alternative to VWR, Pyrex supplier too
* Westnet : local source of Pyrex glassware, good service and cheapest
**registered as new lab [[User:ClarkeS|Sean]] 16:27, 30 June 2006 (EDT), received contract number for up to 70% off list, not posted for privacy
 
* [http://www.fishersci.com Fisher Scientific]
** ordered several pieces of literature [[User:ClarkeS|Sean]] 15:59, 6 July 2006 (EDT)
** registered with website but don't have account yet (phone or fax) [[User:ClarkeS|Sean]] 12:34, 14 July 2006 (EDT)
 
===Equipment===
 
* Eppendorf (centrifuges--tabletop and micro, pipettes, shaking dry baths, PCR machines), Meagan
 
* Torrey Pines (incubators and dry baths)
** rec'd catalog [[User:ClarkeS|Sean]] 17:27, 12 July 2006 (EDT)
 
* PCR machines: ABI for RT, Brinkmann Eppendorf rep = Meagan Hennessey (800-645-3050 x2681 or hennessey.m at eppendorf), Bio-Rad (bought MJ) rep Janusz_Wicher@bio-rad also for protein gel equipment, power supplies
 
===Chemicals/Reagents===


* Westnet : local source of Pyrex glassware, good service and cheapest
* [http://www.sigma.com Sigma] : chemicals as alternative to VWR, Pyrex supplier too
** registered as new lab [[User:ClarkeS|Sean]] 16:27, 30 June 2006 (EDT), received contract number for up to 70% off list, not posted for privacy
** ordered more catalogs for equipment, etc. to add to Sigma and Life Sciences ordered earlier [[User:ClarkeS|Sean]] 15:59, 6 July 2006 (EDT)
** main catalog rec'd [[User:ClarkeS|Sean]] 17:27, 12 July 2006 (EDT)


* NEB/Fermentas : restriction enzymes, they have a set discount for MIT, same-day shipping if order by 10 am, $100 on-line orders get free shipping
* NEB/Fermentas : restriction enzymes, they have a set discount for MIT, same-day shipping if order by 10 am, $100 on-line orders get free shipping
**catalog requested [[User:ClarkeS|Sean]] 16:27, 30 June 2006 (EDT)
** catalog requested [[User:ClarkeS|Sean]] 16:27, 30 June 2006 (EDT)


* Qiagen kits : get through VWR stockroom
* Qiagen kits : get through VWR stockroom or now through Ecat:Sciquest
** ordered catalog [[User:ClarkeS|Sean]] 15:59, 6 July 2006 (EDT)


* Invitrogen: primers, Molecular Probes, free copy of Vector NTI, agarose, dNTPs, RT-PCR kits ''contact steve.smith@invitrogen'' for quote, should be $0.23/bp for 25 nmol scale, $0.37/bp for 50 nmol scale
* Invitrogen: primers, Molecular Probes, free copy of Vector NTI, agarose, dNTPs, RT-PCR kits ''contact steve.smith@invitrogen'' for quote, should be $0.23/bp for 25 nmol scale, $0.37/bp for 50 nmol scale
* C & H for carts and bins
**catalog requested [[User:ClarkeS|Sean]] 16:27, 30 June 2006 (EDT)


* GE Health Care division (formerly Amersham): radioactivity, dNTPs, templiphi
* GE Health Care division (formerly Amersham): radioactivity, dNTPs, templiphi
Line 138: Line 202:


* Ambion : RNA company
* Ambion : RNA company
** requested literature [[User:ClarkeS|Sean]] 17:06, 6 July 2006 (EDT)
* [http://www.macherey-nagel.com Macherey-Nagel] DNA/RNA Purification kits
** contacted US Sales rep, there is also a New England technical rep, they're sending catalog/prices [[User:ClarkeS|Sean]] 15:59, 6 July 2006 (EDT)
* Roche = protein
* Pierce = protein
===Misc===
* C & H for carts and bins
**catalog requested [[User:ClarkeS|Sean]] 16:27, 30 June 2006 (EDT)


* Cole-Parmer: industrial equipment and supplies, fittings, tubing  
* Cole-Parmer: industrial equipment and supplies, fittings, tubing  
Line 144: Line 221:
* Upchurch: chromatography and fluid xfer components
* Upchurch: chromatography and fluid xfer components


* Fisher Scientific
* [http://www.ResearchML.com ML] Unique/hard-to-find useful lab products, have catalog


* Roche = protein


* Pierce = protein
[http://www.hopkinsmedicine.org/cellbio/webengine/webpage.cfm?webpageID=36 Links to many companies at Hopkins site]


PCR machines: ABI for RT, Brinkmann Eppendorf rep = Meagan Hennessey (mhennessey@brinkmann), Bio-Rad (bought MJ) rep Janusz_Wicher@bio-rad also for protein gel equipment, power supplies
=='''Used lab equipment sources'''==
* [[http://www.labx.com labx]] Marketplace, can place wanted ads
* [[http://www.ebay.com ebay]]
* [[http://www.sci-bay.com/store.asp?pid=3244&catid=19596 Sci-Bay]] Surplus/new


Owl gel equipment from VWR
=='''Repairs'''==
[[User:ClarkeS|Sean]]
===shaking thermal block for microtubes===
*PHMT B0608 B0032
*called 3/28/08 Grant Scientific 8009923028
*4/1/08 9084313028/31? Joe Costello will send replacement belt


=='''Used lab equipment sources'''==
[[User:ClarkeS|Sean]]
* [[http://www.labx.com labx]]
===autoclave===
* [[http://www.ebay.com ebay]]
*Ranger, Frank/Tom?
*no cord, no exhaust pipe, rewired to single phase, have to go back to three-phase
*Cambridge Scientific customer service problems

Latest revision as of 07:58, 19 May 2009

Alm:Internal

Hints from a technician in the Endy lab: Basic plasticware (gloves, pipette tips, eppendorf tubes) we get from [ISC Bioexpress]. They are by far the cheapest but to get those prices you have to deal with the company rep. who issues a price quote. USA Scientific (a sister company of Eppendorf) is also a good source for tubes, tips, and racks, and the lower prices are available through ecat3 on MIT's ecat. A lot of people simply use the VWR stockroom in [Building 56] and just go and buy what they need as they need it. You can also just start signing up for accounts with different companies online and start requesting their catalogs so you can get a feel for what they offer. With most companies you can also use Purchase Orders if you prefer or order through webpages with a credit card. Eric has an MIT credit card.


Often-ordered product numbers

S41873 Butane burner canisters (Replacement Fuel For S41872) (Fisher) ($7.85ea + 21.50 hazmat charge) Wall Lenk Vendor No.:65F

21008-959 Eppendorf Safe-Lock 1.5 mL Tubes Natural 022363204 Pack of 500 $34.27

9187-1000 Tough Tags for 1.5ml tubes 1.2x0.5" white (USA)

9185-0500 Tough Spots for 1.5ml tubes 0.5" diam. white (USA)

2344-1000 Tubular 4-way tube racks in set of 4 + tray (USA)

0497735 4 chisel tip dry erase markers (OD)

0254452 Uniball refills black (OD)

32916-536 Large Evolution One gloves, case 500 (VWR)

32916-500 M Diamond Grip, CS 10 boxes (VWR)

60819-761 14ml Falcon (352059) dual-position snap-cap polypropylene culture tubes, case 500 (VWR)

25384-094 case of 600 (25 sleeves of 24) stackable for automation 100x15mm Petri plates (VWR)

73520-774 Nunc large square plates DISH BIOASSAY LO PROFILE case 20 (VWR)

37000-700 galaxy micro centrifuge for spinning down tubes and strips $270 includes rotors/adaptors (VWR)

2004-00004 Silicone closures for flasks, 10 (Bellco Glass, Inc)

14220-010 Autoclave bags, clear with biohazard symbol (available without for $225.83), 12"x24" (slightly bigger?), 1.5 mil polypropylene Case of 1000 $243.64

95042-554 Autoclave bags, clear, 12"x24" 2mil thick polypropylene PK100 $33.05

WLS2493-A PK100 $19.95 VWR BAG AUTOCLAVE 12X24INPK100 Supplier: Tufpak (haven't tried these but they're cheaper)

Vendor numbers

  • Fisher 142706

Miscellaneous

  • Desk chairs: Office Depot (may be cheaper to go through regular site than the SAPwebpage)
    • Herman Miller through procurement office NE49 600 Tech Square 4th floor
  • Lab stools
    • VWR BioFit High Bench height (lab benches require chairs to go up to 29 inches)
    • VWR Urethane lab stool
  • Telephone [MIT's phone service page]
    • All requests for new telephone service or changes in existing service should go through your department's Administrative Officer (AO). Email customer service at telecom-csr@mit.edu or call 617-253-3670 for April-White Frager (Civil's rep).

Lab-wide Equipment

  • Streamline Vertical Laminar FLow Cabinet SCV4A2 2007 20789
  • Eppendorf PCR machine
  • Heat Blocks (dry baths)
  • Stir Plate / Hot Plate
  • Waterbath (s)
  • Incubators
  • -20C Freezer (double-size, used from Broad, Fisher Sci model)
  • 4C Refrigerator (glass front?) (VWR or used)
  • Benchtop Centrifuge for culture tubes
  • Microcentrifuge, Epp unrefrigerated
  • Nanodrop
  • Microbalance (enclosed, for up to __ g)
  • Balance <400g
  • Test tube roller
  • Pressure cooker/hot plate for low cost autoclaving or small autoclave on wheels like on 4th floor

Bench Top Equipment

  • Mini spin, VWR
  • Burners
  • Gas canisters
  • Butane torch and lighters
  • Vortexer
  • Pipette sets (2.5, 20, 100?, 200 and 1000 ul) find out what tips work, get samples)
  • Repeating pipet
  • Pipet man (motorized pipetter)
  • Thermometers (glass for liquids and infra-red for surface measurements)
  • Timer

Disposables/Plasticware

[ISC Bioexpress]

  • Pipette tips (with/without filter)
  • Gloves latex, nitrile
  • PCR tubes/plates
  • Eppendorf Tubes
  • 15 ml Conical Tubes
  • 50 ml conical tubes
  • 14 ml culture tubes with two-position caps
  • VWR brand lab markers
  • Lab Tape (Tape holder)
  • Kimwipes
  • Cryovials
  • Tough Spots
  • Razor Blades
  • Bottle top filters (Westnet)
  • Freezer boxes (-20 and -80)
  • Racks (plastic for various size tubes)
  • Petri Plates (BD/Falcon?)
  • Pasteur Pipettes (and bulbs)

Glassware/Pyrex

Westnet locally (best prices/service), direct from Corning, through Sigma, also maybe available used

  • Erlenmeyer flasks (baffled for cultures and 1 L for making chemical stocks)
  • Graduated Cylinders
  • Media Bottles (we order from 50 ml up to 1 L)
  • Beakers (maybe?)
  • Test tubes

Other items

  • Safety Glasses $7 at VWR
  • Lab coats
  • Test Tubes
  • plastic pipettes
  • Plastic Bins (greatly help with organization)


Chemicals

[Sigma/Aldrich] (bad service, $10 2-day shipping) or VWR

  • Media reagents
  • Antibiotics
  • Protoplast fusion ingredients: EDTA, sodium maleate, etc.
  • EtBr or SYBR Green/Gold/Safe

Enzymes

  • Basic set of restriction enzymes
  • PCR reagents: DNAPols, dNTPs
  • Ligase
  • Qiagen kits

Shared with other labs or borrowed to begin with

  • -80C Freezer (Chisholm)
  • gel reader box and computer, UV gel viewer? (Polz)
  • MilliQ water (Polz/Chisholm/Delong)
  • Other spectrophotometer (or portable A600 spec ~$700)

Suppliers

General

  • VWR stockroom at MIT : most equipment, chemicals, convenient but not necessarily cheapest, can pick up some items in 56-070 or deliver next-day, carry Owl gel equipment
    • ordered catalog Sean 16:27, 30 June 2006 (EDT)
  • USA Scientific Now in Ecat:Sciquest (like Eppendorf just cheaper) : plastic racks, ToughSpot labels, plasticware
  • ISC BioExpress : pipet tips, gloves, plasticware
    • registered and account approved Sean 17:02, 30 June 2006 (EDT)
  • Westnet : local source of Pyrex glassware, good service and cheapest
  • Fisher Scientific
    • ordered several pieces of literature Sean 15:59, 6 July 2006 (EDT)
    • registered with website but don't have account yet (phone or fax) Sean 12:34, 14 July 2006 (EDT)

Equipment

  • Eppendorf (centrifuges--tabletop and micro, pipettes, shaking dry baths, PCR machines), Meagan
  • Torrey Pines (incubators and dry baths)
    • rec'd catalog Sean 17:27, 12 July 2006 (EDT)
  • PCR machines: ABI for RT, Brinkmann Eppendorf rep = Meagan Hennessey (800-645-3050 x2681 or hennessey.m at eppendorf), Bio-Rad (bought MJ) rep Janusz_Wicher@bio-rad also for protein gel equipment, power supplies

Chemicals/Reagents

  • Sigma : chemicals as alternative to VWR, Pyrex supplier too
    • registered as new lab Sean 16:27, 30 June 2006 (EDT), received contract number for up to 70% off list, not posted for privacy
    • ordered more catalogs for equipment, etc. to add to Sigma and Life Sciences ordered earlier Sean 15:59, 6 July 2006 (EDT)
    • main catalog rec'd Sean 17:27, 12 July 2006 (EDT)
  • NEB/Fermentas : restriction enzymes, they have a set discount for MIT, same-day shipping if order by 10 am, $100 on-line orders get free shipping
    • catalog requested Sean 16:27, 30 June 2006 (EDT)
  • Qiagen kits : get through VWR stockroom or now through Ecat:Sciquest
    • ordered catalog Sean 15:59, 6 July 2006 (EDT)
  • Invitrogen: primers, Molecular Probes, free copy of Vector NTI, agarose, dNTPs, RT-PCR kits contact steve.smith@invitrogen for quote, should be $0.23/bp for 25 nmol scale, $0.37/bp for 50 nmol scale
  • GE Health Care division (formerly Amersham): radioactivity, dNTPs, templiphi
  • Stratagene: competent cells, kit for single base mutagenesis, Promega
    • requested catalog Sean 16:49, 30 June 2006 (EDT)
  • Ambion : RNA company
    • requested literature Sean 17:06, 6 July 2006 (EDT)
  • Macherey-Nagel DNA/RNA Purification kits
    • contacted US Sales rep, there is also a New England technical rep, they're sending catalog/prices Sean 15:59, 6 July 2006 (EDT)
  • Roche = protein
  • Pierce = protein

Misc

  • C & H for carts and bins
    • catalog requested Sean 16:27, 30 June 2006 (EDT)
  • Cole-Parmer: industrial equipment and supplies, fittings, tubing
    • requested catalog and registered (just username and pw) Sean 16:49, 30 June 2006 (EDT)
  • Upchurch: chromatography and fluid xfer components
  • ML Unique/hard-to-find useful lab products, have catalog


Links to many companies at Hopkins site

Used lab equipment sources

Repairs

Sean

shaking thermal block for microtubes

  • PHMT B0608 B0032
  • called 3/28/08 Grant Scientific 8009923028
  • 4/1/08 9084313028/31? Joe Costello will send replacement belt

Sean

autoclave

  • Ranger, Frank/Tom?
  • no cord, no exhaust pipe, rewired to single phase, have to go back to three-phase
  • Cambridge Scientific customer service problems
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