Alm:Lab equipment: Difference between revisions

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Hints from a technician in the Endy lab: Basic plasticware (gloves, pipette tips, eppendorf tubes) we get from [[http://www.bioexpress.com ISC Bioexpress]].  They are by far the cheapest but to get those prices you have to deal with the company rep. who issues a price quote.  USA Scientific (a sister company of Eppendorf) is also a good source for plasticware and racks, etc and they issue a blank quote for all of MIT, so you just need to be in touch with the rep to get that quote number but it is only good for certain items.  A lot of people simply use the VWR stockroom in [[http://whereis.mit.edu/map-jpg?mapterms=56&mapsearch=go Building 56]] and just go and buy what they need as they need it.  You can also just start signing up for accounts with different companies online and start requesting their catalogs so you can get a feel for what they offer. With most companies you can also use Purchase Orders if you prefer or order through webpages with a credit card.
Hints from a technician in the Endy lab: Basic plasticware (gloves, pipette tips, eppendorf tubes) we get from [[http://www.bioexpress.com ISC Bioexpress]].  They are by far the cheapest but to get those prices you have to deal with the company rep. who issues a price quote.  USA Scientific (a sister company of Eppendorf) is also a good source for plasticware and racks, etc and they issue a blank quote for all of MIT, so you just need to be in touch with the rep to get that quote number but it is only good for certain items.  A lot of people simply use the VWR stockroom in [[http://whereis.mit.edu/map-jpg?mapterms=56&mapsearch=go Building 56]] and just go and buy what they need as they need it.  You can also just start signing up for accounts with different companies online and start requesting their catalogs so you can get a feel for what they offer. With most companies you can also use Purchase Orders if you prefer or order through webpages with a credit card. Eric has an MIT credit card.
 
We should put through the application for an MIT Visa so we will have a more convenient way of spending from our accounts for things we can't get from SAPWeb or are cheaper somewhere else.




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==Lab-wide Equipment==
==Lab-wide Equipment==
 
*PCR machine (get loaner from Epp, MJ only available used)
*Streamline Vertical Laminar FLow Cabinet SCV4A2 2007 20789
*Heat Block
*Eppendorf PCR machine
*Heat Blocks (dry baths)
*Stir Plate / Hot Plate
*Stir Plate / Hot Plate
*Waterbath (s)
*Waterbath (s)

Revision as of 13:07, 10 December 2007

Hints from a technician in the Endy lab: Basic plasticware (gloves, pipette tips, eppendorf tubes) we get from [ISC Bioexpress]. They are by far the cheapest but to get those prices you have to deal with the company rep. who issues a price quote. USA Scientific (a sister company of Eppendorf) is also a good source for plasticware and racks, etc and they issue a blank quote for all of MIT, so you just need to be in touch with the rep to get that quote number but it is only good for certain items. A lot of people simply use the VWR stockroom in [Building 56] and just go and buy what they need as they need it. You can also just start signing up for accounts with different companies online and start requesting their catalogs so you can get a feel for what they offer. With most companies you can also use Purchase Orders if you prefer or order through webpages with a credit card. Eric has an MIT credit card.


Often-ordered product numbers

9187-1000 Tough Tags for 1.5ml tubes 1.2x0.5" white (USA) 9185-0500 Tough Spots for 1.5ml tubes 0.5" diam. white (USA) 2344-1000 Tubular 4-way tube racks in set of 4 + tray (USA) 0497735 4 chisel tip dry erase markers (OD) 0254452 Uniball refills black (OD)

Miscellaneous

  • Desk chairs: Office Depot (may be cheaper to go through regular site than the SAPwebpage)
    • Herman Miller through procurement office NE49 600 Tech Square 4th floor
  • Lab stools
    • VWR BioFit High Bench height (lab benches are at X inches)
  • Telephone [MIT's phone service page]
    • All requests for new telephone service or changes in existing service should go through your department's Administrative Officer (AO). Email customer service at telecom-csr@mit.edu or call 617-253-3670 for April-White Frager (Civil's rep).

Lab-wide Equipment

  • Streamline Vertical Laminar FLow Cabinet SCV4A2 2007 20789
  • Eppendorf PCR machine
  • Heat Blocks (dry baths)
  • Stir Plate / Hot Plate
  • Waterbath (s)
  • Incubators
  • -20C Freezer (double-size, used from Broad, Fisher Sci model)
  • 4C Refrigerator (glass front?) (VWR or used)
  • Benchtop Centrifuge for culture tubes
  • Microcentrifuge, Epp unrefrigerated
  • Nanodrop
  • Microbalance (enclosed, for up to __ g)
  • Balance <400g
  • Test tube roller
  • Pressure cooker/hot plate for low cost autoclaving or small autoclave on wheels like on 4th floor

Bench Top Equipment

  • Mini spin, VWR
  • Burners
  • Gas canisters
  • Butane torch and lighters
  • Vortexer
  • Pipette sets (2.5, 20, 100?, 200 and 1000 ul) find out what tips work, get samples)
  • Repeating pipet
  • Pipet man (motorized pipetter)
  • Thermometers (glass for liquids and infra-red for surface measurements)
  • Timer

Disposables/Plasticware

[ISC Bioexpress]

  • Pipette tips (with/without filter)
  • Gloves latex, nitrile
  • PCR tubes/plates
  • Eppendorf Tubes
  • 15 ml Conical Tubes
  • 50 ml conical tubes
  • 14 ml culture tubes with two-position caps
  • VWR brand lab markers
  • Lab Tape (Tape holder)
  • Kimwipes
  • Cryovials
  • Tough Spots
  • Razor Blades
  • Bottle top filters (Westnet)
  • Freezer boxes (-20 and -80)
  • Racks (plastic for various size tubes)
  • Petri Plates (BD/Falcon?)
  • Pasteur Pipettes (and bulbs)

Glassware/Pyrex

Westnet locally (best prices/service), direct from Corning, through Sigma, also maybe available used

  • Erlenmeyer flasks (baffled for cultures and 1 L for making chemical stocks)
  • Graduated Cylinders
  • Media Bottles (we order from 50 ml up to 1 L)
  • Beakers (maybe?)
  • Test tubes

Other items

  • Safety Glasses $7 at VWR
  • Lab coats
  • Test Tubes
  • plastic pipettes
  • Plastic Bins (greatly help with organization)


Chemicals

[Sigma/Aldrich] (bad service, $10 2-day shipping) or VWR

  • Media reagents
  • Antibiotics
  • Protoplast fusion ingredients: EDTA, sodium maleate, etc.
  • EtBr or SYBR Green/Gold/Safe

Enzymes

  • Basic set of restriction enzymes
  • PCR reagents: DNAPols, dNTPs
  • Ligase
  • Qiagen kits

Shared with other labs or borrowed to begin with

  • -80C Freezer (Chisholm)
  • gel reader box and computer, UV gel viewer? (Polz)
  • MilliQ water (Polz/Chisholm/Delong)
  • Other spectrophotometer (or portable A600 spec ~$700)

Suppliers

General

  • VWR stockroom at MIT : most equipment, chemicals, convenient but not necessarily cheapest, can pick up some items in 56-070 or deliver next-day, carry Owl gel equipment
    • ordered catalog Sean 16:27, 30 June 2006 (EDT)
  • USA Scientific Now in Ecat:Sciquest (like Eppendorf just cheaper) : plastic racks, ToughSpot labels, plasticware
  • ISC BioExpress : pipet tips, gloves, plasticware
    • registered and account approved Sean 17:02, 30 June 2006 (EDT)
  • Westnet : local source of Pyrex glassware, good service and cheapest
  • Fisher Scientific
    • ordered several pieces of literature Sean 15:59, 6 July 2006 (EDT)
    • registered with website but don't have account yet (phone or fax) Sean 12:34, 14 July 2006 (EDT)

Equipment

  • Eppendorf (centrifuges--tabletop and micro, pipettes, shaking dry baths, PCR machines), Meagan
  • Torrey Pines (incubators and dry baths)
    • rec'd catalog Sean 17:27, 12 July 2006 (EDT)
  • PCR machines: ABI for RT, Brinkmann Eppendorf rep = Meagan Hennessey (800-645-3050 x2681 or hennessey.m at eppendorf), Bio-Rad (bought MJ) rep Janusz_Wicher@bio-rad also for protein gel equipment, power supplies

Chemicals/Reagents

  • Sigma : chemicals as alternative to VWR, Pyrex supplier too
    • registered as new lab Sean 16:27, 30 June 2006 (EDT), received contract number for up to 70% off list, not posted for privacy
    • ordered more catalogs for equipment, etc. to add to Sigma and Life Sciences ordered earlier Sean 15:59, 6 July 2006 (EDT)
    • main catalog rec'd Sean 17:27, 12 July 2006 (EDT)
  • NEB/Fermentas : restriction enzymes, they have a set discount for MIT, same-day shipping if order by 10 am, $100 on-line orders get free shipping
    • catalog requested Sean 16:27, 30 June 2006 (EDT)
  • Qiagen kits : get through VWR stockroom or now through Ecat:Sciquest
    • ordered catalog Sean 15:59, 6 July 2006 (EDT)
  • Invitrogen: primers, Molecular Probes, free copy of Vector NTI, agarose, dNTPs, RT-PCR kits contact steve.smith@invitrogen for quote, should be $0.23/bp for 25 nmol scale, $0.37/bp for 50 nmol scale
  • GE Health Care division (formerly Amersham): radioactivity, dNTPs, templiphi
  • Stratagene: competent cells, kit for single base mutagenesis, Promega
    • requested catalog Sean 16:49, 30 June 2006 (EDT)
  • Ambion : RNA company
    • requested literature Sean 17:06, 6 July 2006 (EDT)
  • Macherey-Nagel DNA/RNA Purification kits
    • contacted US Sales rep, there is also a New England technical rep, they're sending catalog/prices Sean 15:59, 6 July 2006 (EDT)
  • Roche = protein
  • Pierce = protein

Misc

  • C & H for carts and bins
    • catalog requested Sean 16:27, 30 June 2006 (EDT)
  • Cole-Parmer: industrial equipment and supplies, fittings, tubing
    • requested catalog and registered (just username and pw) Sean 16:49, 30 June 2006 (EDT)
  • Upchurch: chromatography and fluid xfer components
  • ML Unique/hard-to-find useful lab products, have catalog


Links to many companies at Hopkins site

Used lab equipment sources

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