Alm:Lab Specific Chemical Hygiene

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This training should be completed on your first day in lab and must be completed before you can start any bench work.  The training takes approximately 30 minutes and consists of a walk through of the lab with the Safety Officer (Sean) in order to introduce you to the space you will be working in.  Please schedule this training with Sean prior to your arrival.  It will cover the following:
This training should be completed on your first day in lab and must be completed before you can start any bench work.  The training takes approximately 30 minutes and consists of a walk through of the lab with the Safety Officer (Sean) in order to introduce you to the space you will be working in.  Please schedule this training with Sean prior to your arrival.  It will cover the following:
-
#No food or drink in lab  
+
#No food, drink, smoking, makeup/Chapstick application in lab
-
#Safety Equipment (Eyewash Stations, Showers, Spill Kits)
+
#Appropriate Lab Clothing: no open-toed shoes, long pants, glasses with flame, coat to protect clothing for dishwashing and EtBr
 +
#Keep door closed
#Emergency Contacts/Flip Charts/Green Sheets
#Emergency Contacts/Flip Charts/Green Sheets
#Dial 100 during an Emergency
#Dial 100 during an Emergency
-
#Personal Protective Equipment/Appropriate Lab Clothing
+
#Personal Protective Equipment/
#Hazardous Chemicals  
#Hazardous Chemicals  
#*Hazards associated with Specific Chemicals we use
#*Hazards associated with Specific Chemicals we use
-
#**EtBr
+
#**EtBr: use nitrile gloves, containment, disposal
#*Proper Disposal
#*Proper Disposal
-
#**Hazardous waste (Satellite Accumulation Area)
+
#**Hazardous waste (Satellite Accumulation Area): gas, EtBr, alcohols
-
#**Sharps
+
#**Sharps: tips, pipets, wooden sticks, blades, Eppis
-
#**Biological waste: liquid/solid
+
#**Biological waste
-
#MSDS and Introduction to Lab Reference Shelf
+
***liquid: add bleach to 10%
-
#Fume hood
+
***solid: autoclave bags
-
#Gas bottles
+
#Safety Equipment (Eyewash Stations, Showers, Spill Kits)
-
#Autoclave Safety
+
#MSDS and Introduction to Lab Reference Shelf (online too)
 +
#Fume hood: keep closed and clear
 +
#Gas bottles: use and disposal
 +
#Autoclave Safety: loosen lids, hot steam, pressure, correct cycle, orange gloves
#BL2 Requirements
#BL2 Requirements
-
#Proper labeling of chemicals
+
#Proper labeling of chemicals (date, initials, contents, few abbreviations, color)
-
#Safety shower/Eye wash stations
+
#Emergency Evacuations/Fire Alarms/Meeting Location across street at Stata
-
#Emergency Evacuations/Fire Alarms/Lab Meeting Location
+
#Fire Extinguishers/MIT’s policy is not to fight fires
#Fire Extinguishers/MIT’s policy is not to fight fires
#Location of First Aid Kit
#Location of First Aid Kit
#Review the EAP (Emergency Action Plan)
#Review the EAP (Emergency Action Plan)
-
#Injury Reporting
+
#Injury--report to PI
 +
 
 +
==Good lab practice==
 +
#cleaning up
 +
#borrowing things, sharing space
 +
#recycling
 +
#no gloves with computer/phone
 +
#laminar flow hood use (UV!)
 +
#centrifuges (balance, covering, changing rotors)
 +
#keep one sink clean
 +
#washing dishes in different sinks
 +
#MQ and DI water
 +
#tools
 +
#microwave
 +
#stocks
 +
#stockroom
 +
#upstairs: MQ, autoclave, ice, Speedvac
 +
 
 +
==Specific trainings==
 +
*pH meter
 +
*electrophoresis
 +
*gels: staining, imaging
 +
*Nanodrop
 +
*balances
 +
*centrifuges
Return to [[Safety Training]] homepage
Return to [[Safety Training]] homepage

Revision as of 10:38, 21 August 2008

Note that this Training must be updated annually.

This training should be completed on your first day in lab and must be completed before you can start any bench work. The training takes approximately 30 minutes and consists of a walk through of the lab with the Safety Officer (Sean) in order to introduce you to the space you will be working in. Please schedule this training with Sean prior to your arrival. It will cover the following:

  1. No food, drink, smoking, makeup/Chapstick application in lab
  2. Appropriate Lab Clothing: no open-toed shoes, long pants, glasses with flame, coat to protect clothing for dishwashing and EtBr
  3. Keep door closed
  4. Emergency Contacts/Flip Charts/Green Sheets
  5. Dial 100 during an Emergency
  6. Personal Protective Equipment/
  7. Hazardous Chemicals
    • Hazards associated with Specific Chemicals we use
      • EtBr: use nitrile gloves, containment, disposal
    • Proper Disposal
      • Hazardous waste (Satellite Accumulation Area): gas, EtBr, alcohols
      • Sharps: tips, pipets, wooden sticks, blades, Eppis
      • Biological waste
      • liquid: add bleach to 10%
      • solid: autoclave bags
  1. Safety Equipment (Eyewash Stations, Showers, Spill Kits)
  2. MSDS and Introduction to Lab Reference Shelf (online too)
  3. Fume hood: keep closed and clear
  4. Gas bottles: use and disposal
  5. Autoclave Safety: loosen lids, hot steam, pressure, correct cycle, orange gloves
  6. BL2 Requirements
  7. Proper labeling of chemicals (date, initials, contents, few abbreviations, color)
  8. Emergency Evacuations/Fire Alarms/Meeting Location across street at Stata
  9. Fire Extinguishers/MIT’s policy is not to fight fires
  10. Location of First Aid Kit
  11. Review the EAP (Emergency Action Plan)
  12. Injury--report to PI

Good lab practice

  1. cleaning up
  2. borrowing things, sharing space
  3. recycling
  4. no gloves with computer/phone
  5. laminar flow hood use (UV!)
  6. centrifuges (balance, covering, changing rotors)
  7. keep one sink clean
  8. washing dishes in different sinks
  9. MQ and DI water
  10. tools
  11. microwave
  12. stocks
  13. stockroom
  14. upstairs: MQ, autoclave, ice, Speedvac

Specific trainings

  • pH meter
  • electrophoresis
  • gels: staining, imaging
  • Nanodrop
  • balances
  • centrifuges

Return to Safety Training homepage

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