Alik:Protocols (Media)
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3T3 media
| 450 mL | Regular DMEM |
| 50 mL (1 tube) | FBS |
| 5 mL | Pen-Strep |
ES media (mouse)
| ~425 mL (416.5 mL to be exact) | Knockout DMEM |
| 75 mL (2 tubes) | ES-FBS |
| 50uL | LIF |
| 1 mL | beta-mercaptoethanol |
| 5 mL | non-essential amino acids |
| 2.5 ml (1 tube) | L-glutamine |
| 5 mL | Pen-Strep |
EB media (mouse)
(Same as ES media, only without Lif)
| ~425 mL (416.5 mL to be exact) | Knockout DMEM |
| 75 mL (2 tubes) | ES-FBS |
| 1 mL | beta-mercaptoethanol |
| 5 mL | non-essential amino acids |
| 2.5 ml (1 tube) | L-glutamine |
| 5 mL | Pen-Strep |
