AhmadWeek3

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==Part 1: Experimenting with different levels of ammonia==
*<i> Saccharomyces cerevisae</i>, a type of yeast, uses ammonia as its primary nitrogen source
*<i> Saccharomyces cerevisae</i>, a type of yeast, uses ammonia as its primary nitrogen source
**Past research has shown that ammonia concentration affects growth
**Past research has shown that ammonia concentration affects growth
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**Glutamate: Increased  
**Glutamate: Increased  
**Glutamine: Increased
**Glutamine: Increased
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*Figure 1
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**X axis: NH4 concentration
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**Y axis:
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***A: Residual NH4 concentration
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***B: 02 consumption/CO2 production
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***C: Ketoglutarate, Glutamate, Glutamine concentrations
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==Part 2: Gene Expression==

Revision as of 02:38, 31 January 2013

Part 1: Experimenting with different levels of ammonia

  • Saccharomyces cerevisae, a type of yeast, uses ammonia as its primary nitrogen source
    • Past research has shown that ammonia concentration affects growth
    • In this paper, the researches wanted to know if ammonia flux, and not concentration, is the limiting factor
  • Physiological Parameters
    • S. cerevisae was first grown in cultures with different levels of ammonia, but fixed glucose concentrations
    • Increases in the ammonia concentration from 29 to 61 mM showed a change in biomass from 4.9 to 8.2 g/L
    • After 61 mM, however, there was no significant increase in biomass
    • Formula to calculate ammonia flux: [dilution x (input ammonia concentration - residual ammonia concentration)/biomass]
    • At a level above 44 mM, oxygen consumption was equal to carbon dioxide production
  • Ketoglutarate, Glutamate, Glutamine concentrations as ammonia concentrations were increased
    • Ketoglutarate: Decreased
    • Glutamate: Increased
    • Glutamine: Increased
  • Figure 1
    • X axis: NH4 concentration
    • Y axis:
      • A: Residual NH4 concentration
      • B: 02 consumption/CO2 production
      • C: Ketoglutarate, Glutamate, Glutamine concentrations

Part 2: Gene Expression

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