AhmadWeek3

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**After 61 mM, however, there was no significant increase in biomass
**After 61 mM, however, there was no significant increase in biomass
**Formula to calculate ammonia flux: [dilution x (input ammonia concentration - residual ammonia concentration)/biomass]
**Formula to calculate ammonia flux: [dilution x (input ammonia concentration - residual ammonia concentration)/biomass]
-
**
+
**At a level above 44 mM, oxygen consumption was equal to carbon dioxide production
 +
*Ketoglutarate, Glutamate, Glutamine concentrations as ammonia concentrations were increased
 +
**Ketoglutarate: Decreased
 +
**Glutamate: Increased
 +
**Glutamine: Increased

Revision as of 21:46, 30 January 2013

  • Saccharomyces cerevisae, a type of yeast, uses ammonia as its primary nitrogen source
    • Past research has shown that ammonia concentration affects growth
    • In this paper, the researches wanted to know if ammonia flux, and not concentration, is the limiting factor
  • Physiological Parameters
    • S. cerevisae was first grown in cultures with different levels of ammonia, but fixed glucose concentrations
    • Increases in the ammonia concentration from 29 to 61 mM showed a change in biomass from 4.9 to 8.2 g/L
    • After 61 mM, however, there was no significant increase in biomass
    • Formula to calculate ammonia flux: [dilution x (input ammonia concentration - residual ammonia concentration)/biomass]
    • At a level above 44 mM, oxygen consumption was equal to carbon dioxide production
  • Ketoglutarate, Glutamate, Glutamine concentrations as ammonia concentrations were increased
    • Ketoglutarate: Decreased
    • Glutamate: Increased
    • Glutamine: Increased
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