AhmadWeek3
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(Difference between revisions)
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**After 61 mM, however, there was no significant increase in biomass | **After 61 mM, however, there was no significant increase in biomass | ||
**Formula to calculate ammonia flux: [dilution x (input ammonia concentration - residual ammonia concentration)/biomass] | **Formula to calculate ammonia flux: [dilution x (input ammonia concentration - residual ammonia concentration)/biomass] | ||
| - | ** | + | **At a level above 44 mM, oxygen consumption was equal to carbon dioxide production |
| + | *Ketoglutarate, Glutamate, Glutamine concentrations as ammonia concentrations were increased | ||
| + | **Ketoglutarate: Decreased | ||
| + | **Glutamate: Increased | ||
| + | **Glutamine: Increased | ||
Revision as of 22:46, 30 January 2013
- Saccharomyces cerevisae, a type of yeast, uses ammonia as its primary nitrogen source
- Past research has shown that ammonia concentration affects growth
- In this paper, the researches wanted to know if ammonia flux, and not concentration, is the limiting factor
- Physiological Parameters
- S. cerevisae was first grown in cultures with different levels of ammonia, but fixed glucose concentrations
- Increases in the ammonia concentration from 29 to 61 mM showed a change in biomass from 4.9 to 8.2 g/L
- After 61 mM, however, there was no significant increase in biomass
- Formula to calculate ammonia flux: [dilution x (input ammonia concentration - residual ammonia concentration)/biomass]
- At a level above 44 mM, oxygen consumption was equal to carbon dioxide production
- Ketoglutarate, Glutamate, Glutamine concentrations as ammonia concentrations were increased
- Ketoglutarate: Decreased
- Glutamate: Increased
- Glutamine: Increased
