AhmadWeek11
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Week 11 Journal Assignment
Biological Terms
- Motif- The smallest group of atoms in a polymer that, when under the influence of a rotation-translation operator, will assemble the rest of the atoms in the chain. [1]
- Biogenesis-The process in which life forms arise from similar life forms; living things can only be produced by another living thing, and not by a non-living thing. [2]
- Batch Culture- large-scale closed system culture in which cells are grown in a fixed volume of nutrient culture medium under specific environmental conditions (In this case: nutrient limits, temperature) up to a certain density in a tank or airlift fermentor, harvested and processed as a batch, especially before all nutrients are used up. [3]
- Catabolite- Product of catabolism, the breakdown of complex molecules into simpler ones. [4]
- Trehalose- a crystalline disaccharide (C12H22O11) that is found in various organisms that is about half as sweet as sucrose, and is sometimes used as a sweetener in commercially prepared foods [5]
- Gene Regulatory Protein- Any protein that interacts with dna sequences of a gene and controls its transcription. [6]
- Transcriptome- the complement of mature messenger RNAs produced in a given cell in a given moment of its life. [7]
- Up-regulation- process that increases ligand/receptor interactions due to an increase in the number of available receptors. [8]
- Down-regluation- the process that decreases ligand and receptor interactions or reduces the responsiveness of a cell to a stimulus following first exposure.[9]
- Mannoprotein- yeast cell wall components that are proteins with large numbers of mannose groups attached; highly antigenic." [10]
Outline: Acclimation of Saccharomyces cerevisiae to Low Temperature: A Chemostat-based Transcriptome Analysis
- Background Information
- Experiment
- Dilution Rate: 0.03/h
- Temperature: One set at 12°C and another at 30°C
- Yeast grown in a steady-state chemostat instead of batches that had been used in previous experiments
- Anaerobic conditions
- Haploid yeast
- The two limited nutrients were glucose and ammonia each done separately at different temperatures making 4 data sets
- Three replicas were done for each of the four conditions
- Results were compared to results from previous batch experiments
- In previous experiments, yeast were exposed to cold shock; in this experiment yeast were grown at a lower temperature
- Adaptation vs. Acclimation
Assignments
- Class Journal Week 1
- Journal Assignment Week 2
- Class Journal Week 2
- Journal Assignment Week 3
- Class Journal Week 3
- Journal Assignment Week 4
- Class Journal Week 4
- Journal Assignment Week 5
- Class Journal Week 5
- Journal Assignment Week 6
- Class Journal Week 6
- Journal Assignment Week 8
- Class Journal Week 8
- Journal Assignment Week 9
- Class Journal Week 9
- Journal Assignment Week 11
- Class Journal Week 11
- Journal Assignment Week 12
- Class Journal Week 12
- Journal Assignment Week 13
- Class Journal Week 13
- Assignment Week 13
- Journal Assignment Week 14
- Class Journal Week 14
- Assignment Week 14