Agarose gel electrophoresis

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* [[TAE]] - better resolution of fragments >4kb;
* [[TAE]] - better resolution of fragments >4kb;
* [[TBE]] - better resolution of 0.1-3kb fragments; TBE is better suited for high-voltage (>150V) electrophoresis than TAE because of its higher buffering capacity and lower conductivity;
* [[TBE]] - better resolution of 0.1-3kb fragments; TBE is better suited for high-voltage (>150V) electrophoresis than TAE because of its higher buffering capacity and lower conductivity;
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== Loading dyes ==
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{| cellpadding=5 style="border: 1px solid #CC9;"
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| dye
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| 0.5-1.5% agarose
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| 2.0-3.0% agarose*
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|-
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| xylene cyanol
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| 4000-5000 bp
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| 750 bp
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|-
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| bromophenol blue
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| 400-500 bp
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| 100 bp
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|}
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 +
<nowiki>*</nowiki>sieving agarose
== Specific Protocols ==
== Specific Protocols ==

Revision as of 07:34, 28 June 2006

Contents

General Information

General Procedure

  1. Cast a gel
  2. Place it in gel box in running buffer
  3. Load samples
  4. Run the gel
  5. Image the gel

Buffers

  • TAE - better resolution of fragments >4kb;
  • TBE - better resolution of 0.1-3kb fragments; TBE is better suited for high-voltage (>150V) electrophoresis than TAE because of its higher buffering capacity and lower conductivity;

Loading dyes

dye 0.5-1.5% agarose 2.0-3.0% agarose*
xylene cyanol 4000-5000 bp 750 bp
bromophenol blue 400-500 bp 100 bp

*sieving agarose

Specific Protocols

Endy:Agarose gel

Knight:Agarose gel electrophoresis

Notes

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