Agarose gel electrophoresis

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(Notes)
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==Notes==
==Notes==
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If you are getting unexpected bands on your gel you may want to look at the [[Common Agarose Gel Issues]]
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*If you are getting unexpected bands on your gel you may want to look at the [[Common Agarose Gel Issues]]
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*If you have no experience with gel electrophoresis or are explaining it to someone new, [http://arbl.cvmbs.colostate.edu/hbooks/genetics/biotech/gels/virgel.html here] is a cute java demo of what happens.

Revision as of 11:41, 3 January 2006

Contents

General Information

General Procedure

  1. Cast a gel
  2. Place it in gel box in running buffer
  3. Load samples
  4. Run the gel
  5. Image the gel

Specific Protocols

Endy:Agarose gel

Knight:Agarose gel

Notes

  • If you are getting unexpected bands on your gel you may want to look at the Common Agarose Gel Issues
  • If you have no experience with gel electrophoresis or are explaining it to someone new, here is a cute java demo of what happens.
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