Addition of 3' A overhangs to PCR products: Difference between revisions
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==Abstract== | ==Abstract== | ||
If PCR is performed using a proofreading DNA polymerase, such as ''Pfu'' DNA polymerase, the product will have blunt ends. ''Taq'' DNA polymerase catalyzes the non-template directed addition of an adenine residue to the 3´-end of both strands of DNA molecules to make it suitable for TA cloning. | |||
==Materials== | ==Materials== | ||
Revision as of 20:35, 18 October 2008
Curators
Mohsen Hosseini
Abstract
If PCR is performed using a proofreading DNA polymerase, such as Pfu DNA polymerase, the product will have blunt ends. Taq DNA polymerase catalyzes the non-template directed addition of an adenine residue to the 3´-end of both strands of DNA molecules to make it suitable for TA cloning.
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