Acetobacter Xylinum Culture - Revision history

Matthew Orton: /* Preparation of Acetobacter Media */

2011-04-08T20:23:07Z

Preparation of Acetobacter Media

←Older revision		Revision as of 20:23, 8 April 2011
Line 6:		Line 6:

	To prepare ∼500 ml of liquid Acetobacter media, add the following:		To prepare ∼500 ml of liquid Acetobacter media, add the following:
-	*Glucose - ~~1.0~~ g	+	*Glucose - 10 g
	*Peptone - 2.5 g		*Peptone - 2.5 g
	*Yeast extract - 2.5 g		*Yeast extract - 2.5 g

Matthew Orton: /* Preparation of Acetobacter Media */

2010-03-18T21:12:32Z

Preparation of Acetobacter Media

←Older revision		Revision as of 21:12, 18 March 2010
Line 12:		Line 12:
	*Citric acid - 0.75 g		*Citric acid - 0.75 g
	*Distilled water - 500 ml		*Distilled water - 500 ml
-	*If you are making plates, use the same protocol but add 3.0 g of agar.	+	*If you are making plates, use the same protocol but add 7.5 g of agar.

	=='''Procedure'''==		=='''Procedure'''==

Matthew Orton: /* Notes */

2009-10-19T15:45:56Z

Notes

←Older revision		Revision as of 15:45, 19 October 2009
Line 25:		Line 25:
	All questions, input and feedback are welcome!		All questions, input and feedback are welcome!
	#The growth of Acetobacter does not give a cloudy appearance in the media, the media will remain transparent to slightly translucent in appearance.		#The growth of Acetobacter does not give a cloudy appearance in the media, the media will remain transparent to slightly translucent in appearance.
-	#The growth of Acetobacter is accompanied by the formation of a thick cellulose matrix within the media that must be removed before cells can be pelleted for a miniprep procedure. Simply vortex briefly to break up the cellulose and remove the cellulose from the media with a pipette while carefully avoiding the removal of cells.	+	#The growth of Acetobacter is accompanied by the formation of a thick cellulose matrix within the media that must be removed before cells can be pelleted for a miniprep procedure. Simply vortex briefly to break up the cellulose into chunks and remove the cellulose chunks from the media with a pipette while carefully avoiding the removal of cells.
	#Acetobacter will grow well at room temperature in aerobic conditions.		#Acetobacter will grow well at room temperature in aerobic conditions.
	#For information on the growth conditions of other Acetobacter strains, please visit [http://www.http://www.atcc.org/ATCCAdvancedCatalogSearch/AllCollectionSearch/tabid/454/Default.aspx]		#For information on the growth conditions of other Acetobacter strains, please visit [http://www.http://www.atcc.org/ATCCAdvancedCatalogSearch/AllCollectionSearch/tabid/454/Default.aspx]

Matthew Orton: New page: ==Overview== General guidelines on how to grow up a culture of ''Acetobacter xylinum'', ATCC strain 53582 . =='''Preparation of Acetobacter Media'''== To prepare ∼500 ml of liquid Ace...

2009-10-18T16:38:55Z

New page: ==Overview== General guidelines on how to grow up a culture of ''Acetobacter xylinum'', ATCC strain 53582 . =='''Preparation of Acetobacter Media'''== To prepare ∼500 ml of liquid Ace...

New page

==Overview==

General guidelines on how to grow up a culture of ''Acetobacter xylinum'', ATCC strain 53582 .

=='''Preparation of Acetobacter Media'''==

To prepare ∼500 ml of liquid Acetobacter media, add the following:
*Glucose - 1.0 g
*Peptone - 2.5 g
*Yeast extract - 2.5 g
*Na2HPO4 - 1.35 g
*Citric acid - 0.75 g
*Distilled water - 500 ml
*If you are making plates, use the same protocol but add 3.0 g of agar.

=='''Procedure'''==

# Prepare media as outlined
# Autoclave to sterilize media.
# Streak/inoculate Acetobacter onto plates or in media.
# Incubate cells at 26°C for 2-3 days.
# If using a freeze dried source of Acetobacter (ex. ATCC shipment), growth may take up to 4 days.

==Notes==
All questions, input and feedback are welcome!
#The growth of Acetobacter does not give a cloudy appearance in the media, the media will remain transparent to slightly translucent in appearance.
#The growth of Acetobacter is accompanied by the formation of a thick cellulose matrix within the media that must be removed before cells can be pelleted for a miniprep procedure. Simply vortex briefly to break up the cellulose and remove the cellulose from the media with a pipette while carefully avoiding the removal of cells.
#Acetobacter will grow well at room temperature in aerobic conditions.
#For information on the growth conditions of other Acetobacter strains, please visit [http://www.http://www.atcc.org/ATCCAdvancedCatalogSearch/AllCollectionSearch/tabid/454/Default.aspx]

==Contact==
*morto077@uottawa.ca

or instead, [[Talk:{{PAGENAME}}|discuss this protocol]].



[[Category:Protocol]]

[[Category:In vivo]]

[[Category:Acetobacter xylinum]]
-->