AU Biomaterials Design Lab:Protocols/Quantum Northwest Cuvette Holder: Difference between revisions

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(New page: ==Description== The Quantum Northwest Cuvette Holder temperature controller, is able to maintain a specific temperature for your cuvette over the course of an experiment and can also provi...)
 
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==Protocol==
==Protocol==
# Extra Steps
# Fill the clear 8 quart/7.5 L bucket 3/4 full with water
## Do you need temperature control or need stirring?
# Make sure that the aquarium pump is properly connected and inside of the bucket. Plug in the pump.
### See the protocol for controlling the cuvette controller
# Plug in the cuvette controller
# Turn on the jaz spectrophotometer (red on-off button)
# Make sure the computer is turned on
# Turn on the Lamps (Ocean Optics DH-2000)
# Open the "Q Blue Wireless Temperature Controller" by clicking its icon on the desktop
## Turn on the switch in the back of the controller box
# Set the Temperature
## Press the blue "Deuterium" button and wait for the light to stay on
## Set the Control Status to "On"
## Press the red "Halogen" button
## Input your desired temperature
## The lamps need 15 minutes to warm up before you are ready to proceed to the next step.  
### Click "Change" for the Target Temperature and type in the temperature you want for the experiment
# Starting the software
### For most nanoparticle syntheses, the temperature is 80C
## Open the OceanView software.  
# Set the stirring
### Click on the "Create New Spectroscopy Application" icon (looks like a bunch of shark fins stacked on one another)
## If you need stirring, and have a stir bar in your cuvette, set the stirrer to "On"
#### Click on "Spectroscopy"
#### Select JAZA1666 and click "Next"
#### Select "Absorbance" and click "Next"
#### Set Acquisition Parameters
##### Set "Integration Time" to 1 ms
##### Set "Scans to Average" to 100
##### Click "Next"
#### Store Reference Spectrum
##### Make sure that the toggle on the light source is switched to "Open"
##### Click on the light bulb icon
###### A spectrum should appear in the middle display
##### Click "Next"
#### Store Background Spectrum
##### Make sure that the toggle on the light source is switched to "Closed"
##### Click on the light bulb icon
###### A spectrum should appear in the middle display
##### Click "Finish"
#### Make sure that the toggle on the light source is switched to "On"
# Set up your data acquisition schedule
## Click on the "Save graph to file/files" icon (the floppy disc icon on the lower icon panel)
## Click "Yes" for "You must configure save parameters <b>first</b>. Do you want to configure now?"
## Set Save Options
### Choose the "Between saved scans, wait at least" option
### Set the time to whatever the experiment requires
#### For most nanoparticle syntheses, this will be 2 minutes
### Choose the "Stop after this amount of time" option
#### For most nanoparticle syntheses, this will be 3 hours
## Set File Options
### Save to Directory: C:\Users\Lab Admin\DropBox\CHEM471 2016\Year\Month\Date
### Select Open
### Set Basename to something that will be descriptive of your data for example Lysozyme_AuNP_Ratio45_80C
### Click "Apply"
### Click "Exit"
# Start data collection
## After you have your sample, cuvette, and cuvette holder properly prepared perform the following:
### Click the "Save graph to file/files" icon (the floppy disc icon on the lower icon panel)
### Data files should be showing up in your selected folder. If it isn't, you have done something wrong.


==Notes==
==Notes==

Revision as of 13:02, 24 August 2016

Description

The Quantum Northwest Cuvette Holder temperature controller, is able to maintain a specific temperature for your cuvette over the course of an experiment and can also provide a magnetic stirring function, should you need it. This instrumentation is coupled to the Ocean Optics spectrophotometer found in Beeghly 203B and is maintained by Dr. Hartings.

Protocol

  1. Fill the clear 8 quart/7.5 L bucket 3/4 full with water
  2. Make sure that the aquarium pump is properly connected and inside of the bucket. Plug in the pump.
  3. Plug in the cuvette controller
  4. Make sure the computer is turned on
  5. Open the "Q Blue Wireless Temperature Controller" by clicking its icon on the desktop
  6. Set the Temperature
    1. Set the Control Status to "On"
    2. Input your desired temperature
      1. Click "Change" for the Target Temperature and type in the temperature you want for the experiment
      2. For most nanoparticle syntheses, the temperature is 80C
  7. Set the stirring
    1. If you need stirring, and have a stir bar in your cuvette, set the stirrer to "On"

Notes

References

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