AU Biomaterials Design Lab:Equipment/UV-2550 Shimadzu
- Shimadzu® UV-2550 is a ultra-low stray light double monochromator instrument. It provides UV-vis spectral analysis for samples by the embedded double blazed grating double monochromator. High energy optical system, wide dynamic range, small beam size, law stray light are some of its advantages over other double monochromators.
- Its window-compatible software: UVProbe contains three modes: Spectrum, Kinetics, and Photometric modes, that allows wide range of applications.
Purpose of use
- With the current multicell Sample Compartment, liquid and solid samples can be run by inserting into a quartz or plastic cuvette that is able to fit inside multicell Sample Compartment.
- Liquid samples covers wide range including biological and non-biological liquid solutions. Small solid samples can be cut; size of solid samples can be adjusted to fit inside a quartz or plastic cuvette for analysis.
- Instrument is able to perform test on larger sample with the usage of sample compartment that accommodate larger sizes.
- Beeghly 207
- American University
- Washington, DC, 20016
- access to the laboratory is restricted; see Dr. Miller, Dr. Hartings or Dr. Fox for access.
- Instrument has three different methods for analyzing samples: Spectrum, Photometric, and Kinetic methods. The usage for methods are different.
- When instrument is turned on, lick connect on UVProve software to connect instrument with program.
- After instruments calibrated and self checked, proceed to methods.
- Spectrum mode is used for scanning a range of wavelength onto a sample.
- Include any relevant links to OWW protocols
- Include any relevant links to the manufacturer's protocols
- Do not open the cover of instrument while running. To avoid electric shock, first switch off power, then open instrument.
- Do not touch or approach instrument too close. Instrument might overheat leading to possible burns on body at small distance or upon contact.
TCC-240A Thermoelectrically Temperature Controlled Cell Holder
- Uses Peltier effect for controlling the temperatures of the sample and reference sample. No thermostatic bath or cooling water is required, so the operation is quite simple and easy.
- Number of cells: One each on the sample and reference sides.
- Temperature control range: 7 to 60°C
- Temperature display accuracy (difference from the true value): ±0.5°C
- Temperature control precision (variation of temperature): ±0.1°C
Multicell Sample Compartment
- Holds up to six 10 mm square cells. No temperature control capability.
- Number of cells: 6 on the sample side, and 1 on the reference side
- Allows data processing with customized paramaters
- Multitasking (possible to execute data processing while measurement is being executed)
- Customizable measurement screen layout (wavelengths, data display font and font size, colors, displayed number of rows)
- GLP/GMP compliant (security, history)
- Real-time concentration display
Setting Wavelength Range
- 190nm to 1100nm
Measurement Wavelength Range
- 190nm to 900nm, but can measure up to 1100 with choice with detector
Wavelength Scanning Speed
- Wavelength slew rate: about 3200nm/min
- Wavelength scan rate: about 900nm-160nm/min
- Monitor scan rate: about 2500nm/min
- At 1nm units for scan start and sea end wavelengths, and 0.1nm units for over wavelengths
Lamp Interchange wavelength
- Auto switching synchronized with wavelength, switching range selectable between 282nm-393nm (0.1nm units)
- 6-step switching along 0.1/0.2/0.5/1/2/5nm
- Optimum response speed automatically set depending on bandwidth, minimum 0.1sec
- Less than 0.0003% (220nm, Nal 10g/L solution)
- Less than 0.0003% (340nm, UV-39 filter)
- Double-beam, direct-ratio system with dynode feedback
- Absorbance(Abs.), transmittance(%), reflectance(%), energy(E)
- Absorbance: -4 to 5 Abs
- Transmittance, reflectance: 0.0 to 999.9%
- ±0.002 Abs (0-0.5 Abs)
- ±0.004 Abs (0.5-1.0 Abs)
- ±0.3%T (0-100% T) filter
- Tested with NIST 903D standard
- ±0.001 Abs (0-0.5 Abs)
- ±0.002 Abs (0.5-1.0 Abs)
- ±0.001 Abs (excluding noise, using 2nm slit, and slow wavelength scanning speed)
- Auto correction using PC (stored baseline is automatically loaded when power is switched on, re-correction is possible)
- 0.0004Abs/h (after power is on for 2 hours)
Temperature and humidity requirements
- 15-35°C, 45-80%(no condensation, less than 70% above 30°C)
- 50W halogen lamp (2,000 hours life), deuterium lamp (socket type), light source auto position adjustment built in.
- Grating/Grating type double monochromator
- Pre-monochromator: double-blazed holographic grating
- Main monochromator: high-performance blazed holographic grating in aberration-corrected Czerny-Turner mounting
- R928 Red-Sensitive Photomultiplier
- Internal dimensions: 150W x 260D x 120H (mm)
- Distance between light beams: 100nm
- Maximum light path length of cell: 100nm
- AC100, 120, 220, 240V, switch selectable
- 50/60Hz; 250 VA
- 570W x 660D x 275H (mm)
- About 36kg