AU Biomaterials Design Lab:Equipment/UV-2550 Shimadzu - Revision history

Keyun Wang at 23:03, 30 November 2012

2012-11-30T23:03:42Z

←Older revision		Revision as of 23:03, 30 November 2012
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	==Description==		==Description==
		+	[[Image:Photo AU UV 2550 Shimadzu.JPG\|400px\|right\|Shimadzu UV-2550]]
	*Shimadzu® UV-2550 is a ultra-low stray light double monochromator instrument. It provides UV-vis spectral analysis for samples by the embedded double blazed grating double monochromator. High energy optical system, wide dynamic range, small beam size, law stray light are some of its advantages over other double monochromators.		*Shimadzu® UV-2550 is a ultra-low stray light double monochromator instrument. It provides UV-vis spectral analysis for samples by the embedded double blazed grating double monochromator. High energy optical system, wide dynamic range, small beam size, law stray light are some of its advantages over other double monochromators.
	*Its window-compatible software: UVProbe contains three modes: Spectrum, Kinetics, and Photometric modes, that allows wide range of applications.		*Its window-compatible software: UVProbe contains three modes: Spectrum, Kinetics, and Photometric modes, that allows wide range of applications.

Keyun Wang: /* Use */

2012-11-30T22:30:37Z

Use

←Older revision		Revision as of 22:30, 30 November 2012
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	* Additional data points are shown after each sample run.		* Additional data points are shown after each sample run.
	* Graph of absorbance versus time is shown under graphical section of screen.		* Graph of absorbance versus time is shown under graphical section of screen.
-		+	<br>
-	* A copy of the instrument user manual is within the laboratory draw in room 207. See contact for more information.	+	A copy of the instrument user manual is within the laboratory draw in room 207. See contact for more information.

	==Safety==		==Safety==

Keyun Wang: /* Use */

2012-11-30T22:30:02Z

Use

←Older revision		Revision as of 22:30, 30 November 2012
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	* Graph of absorbance versus time is shown under graphical section of screen.		* Graph of absorbance versus time is shown under graphical section of screen.

-	* ~~All files can be saved and be exported into other software~~ for ~~additional analysis~~.	+	* A copy of the instrument user manual is within the laboratory draw in room 207. See contact for more information.
-		+
-	*''Include any relevant links to [[Protocols\|OWW protocols]]''	+
-	*''Include any relevant links to the manufacturer's protocols''	+

	==Safety==		==Safety==

Keyun Wang: /* Purpose of use */

2012-11-30T22:28:53Z

Purpose of use

←Older revision		Revision as of 22:28, 30 November 2012
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	==Purpose of use==		==Purpose of use==
	'''Instrumentation'''		'''Instrumentation'''
-	*	+	*Shimadzu™ UV-2550® Spectrophotometer is used to measure spectrum of light absorption with wavelength ranging from 200nm to 1100nm for liquid, gas, and solid samples. However, since this instrument is only equipped with multicell Sample Compartment, the samples measured can only be liquid or solids.

	'''Sample Range'''		'''Sample Range'''

Keyun Wang: /* Useful links */

2012-11-29T16:17:39Z

Useful links

←Older revision		Revision as of 16:17, 29 November 2012
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	*[[AU_Biomaterials_Design_Lab\| AU Biomaterials Design Lab]]		*[[AU_Biomaterials_Design_Lab\| AU Biomaterials Design Lab]]
	*[http://www.american.edu/cas/chemistry American University Chemistry]		*[http://www.american.edu/cas/chemistry American University Chemistry]
-	*[[~~Category~~:Equipment\|Equipment]]	+	*[[AU_Biomaterials_Design_Lab:Equipment\|Category: Equipment]]

	<!-- *Please replace the second category (Miscellaneous) with one or more category tags that identifies where in the Equipment hierarchy this page belongs. Remove the 'Miscellaneous' category to keep it from ending back up in the list of structural pages that make up that category. -->		<!-- *Please replace the second category (Miscellaneous) with one or more category tags that identifies where in the Equipment hierarchy this page belongs. Remove the 'Miscellaneous' category to keep it from ending back up in the list of structural pages that make up that category. -->

Keyun Wang: /* Useful links */

2012-11-29T16:16:42Z

Useful links

←Older revision		Revision as of 16:16, 29 November 2012
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	*[[AU_Biomaterials_Design_Lab\| AU Biomaterials Design Lab]]		*[[AU_Biomaterials_Design_Lab\| AU Biomaterials Design Lab]]
	*[http://www.american.edu/cas/chemistry American University Chemistry]		*[http://www.american.edu/cas/chemistry American University Chemistry]
-	*[[Category:Equipment]]	+	*[[Category:Equipment\|Equipment]]

	<!-- *Please replace the second category (Miscellaneous) with one or more category tags that identifies where in the Equipment hierarchy this page belongs. Remove the 'Miscellaneous' category to keep it from ending back up in the list of structural pages that make up that category. -->		<!-- *Please replace the second category (Miscellaneous) with one or more category tags that identifies where in the Equipment hierarchy this page belongs. Remove the 'Miscellaneous' category to keep it from ending back up in the list of structural pages that make up that category. -->

Keyun Wang: /* Use */

2012-11-29T05:48:01Z

Use

←Older revision		Revision as of 05:48, 29 November 2012
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	* Instrument has three different methods for analyzing samples: Spectrum, Photometric, and Kinetic methods. The usage for methods are different.		* Instrument has three different methods for analyzing samples: Spectrum, Photometric, and Kinetic methods. The usage for methods are different.
	* When instrument is turned on, lick connect on UVProve software to connect instrument with program.		* When instrument is turned on, lick connect on UVProve software to connect instrument with program.
-	* After instruments calibrated and self checked, proceed to methods.	+	* After instruments calibrated and self checked, proceed to toolbar to select methods.
		+	* Samples can be placed into multicell sample compartment. Door to sample compartment should be closed before samples are run.

	'''Spectrum Method'''		'''Spectrum Method'''
-	* Spectrum mode is used for scanning a range of wavelength onto a sample.	+	* Spectrum mode is used for scanning a range of wavelength onto a sample and can be selected on toolbar.
		+	* M buttom in PVProbe sets up the acquired parameters.
		+	* Place sample into sample compartment. Standard samples in reference sides are preferred.
		+	* Click start to start the run.
		+	* Table is shown be clicking on Data Table, peaks in spectrum can be labeled by clicking on peak pick table.

		+	'''Photometric Method'''
		+	* Photometric mode is used for measuring absorbance at a specific wavelength.
		+	* After parameters are set up in "Method", click on photometric method button in tool bar.
		+	* Select wavelength specified for sample run. Concentration units, type of standard curve can be set up afterwards.
		+	* Then enter measurement parameters for standards. The measurements are acquired by instrument.
		+	* Set sample measurement parameters. Measurement acquired by instrument.
		+	* Standard table is then shown. Fill in the concentration in standard table.
		+	* Load sample.
		+	* Click start to run sample.
		+	* Once data are collected, additional data points are going to shown on standard table.
		+
		+	'''Kinetics Method'''
		+	* Knetics mode collects data at a specific wavelength over a set amount of time.
		+	* After instrument warmed up, select Kinetics Method in toolbar.
		+	* Set up the amount of time for samples to run, the amount of time each data point is taken throughout each run, and the range of wavelength the instrument should run samples at under method section.
		+	* Load sample in sample compartment.
		+	* Click start to run.
		+	* Additional data points are shown after each sample run.
		+	* Graph of absorbance versus time is shown under graphical section of screen.
		+
		+	* All files can be saved and be exported into other software for additional analysis.

	*''Include any relevant links to [[Protocols\|OWW protocols]]''		*''Include any relevant links to [[Protocols\|OWW protocols]]''

Keyun Wang: /* Use */

2012-11-29T05:08:12Z

Use

←Older revision		Revision as of 05:08, 29 November 2012
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	==Use==		==Use==
-	*~~''How does one use this~~ instrument.''	+	* Instrument has three different methods for analyzing samples: Spectrum, Photometric, and Kinetic methods. The usage for methods are different.
		+	* When instrument is turned on, lick connect on UVProve software to connect instrument with program.
		+	* After instruments calibrated and self checked, proceed to methods.
		+
		+	'''Spectrum Method'''
		+	* Spectrum mode is used for scanning a range of wavelength onto a sample.
		+
		+
	*''Include any relevant links to [[Protocols\|OWW protocols]]''		*''Include any relevant links to [[Protocols\|OWW protocols]]''
	*''Include any relevant links to the manufacturer's protocols''		*''Include any relevant links to the manufacturer's protocols''
-	*Note to users: you may note any troubleshooting or log your use on the [[{{TALKPAGENAME}}\|talk page]].

	==Safety==		==Safety==

Keyun Wang: /* Description */

2012-11-29T03:02:40Z

Description

←Older revision		Revision as of 03:02, 29 November 2012
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	==Description==		==Description==
-	*Shimadzu® UV-2550	+	*Shimadzu® UV-2550 is a ultra-low stray light double monochromator instrument. It provides UV-vis spectral analysis for samples by the embedded double blazed grating double monochromator. High energy optical system, wide dynamic range, small beam size, law stray light are some of its advantages over other double monochromators.
-	*Shimadzu® UV-2550 is a ultra-low stray light double monochromator instrument. It provides UV-vis spectral analysis for samples by the embedded double blazed grating double monochromator. High energy optical system, wide dynamic range, small beam size, law stray light are some of its advantages over other double monochromators.	+	*Its window-compatible software: UVProbe contains three modes: Spectrum, Kinetics, and Photometric modes, that allows wide range of applications.

	==Purpose of use==		==Purpose of use==

Keyun Wang: /* Purpose of use */

2012-11-29T02:58:05Z

Purpose of use

←Older revision		Revision as of 02:58, 29 November 2012
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	==Purpose of use==		==Purpose of use==
	'''Instrumentation'''		'''Instrumentation'''
		+	*

	'''Sample Range'''		'''Sample Range'''
		+	*With the current multicell Sample Compartment, liquid and solid samples can be run by inserting into a quartz or plastic cuvette that is able to fit inside multicell Sample Compartment.
		+	*Liquid samples covers wide range including biological and non-biological liquid solutions. Small solid samples can be cut; size of solid samples can be adjusted to fit inside a quartz or plastic cuvette for analysis.
		+	*Instrument is able to perform test on larger sample with the usage of sample compartment that accommodate larger sizes.

	==Location==		==Location==