AU Biomaterials Design Lab:Equipment/Shimadzu AA-6200 - Revision history

Melissa Novy: /* Description */

2012-12-08T04:43:50Z

Description

←Older revision		Revision as of 04:43, 8 December 2012
Line 4:		Line 4:
	* It can detect the concentration of Au and Cu even at very low concentrations (ppm).		* It can detect the concentration of Au and Cu even at very low concentrations (ppm).
	** The limit of detection for Cu absorption is 0.003 ppm.		** The limit of detection for Cu absorption is 0.003 ppm.
-	** The limit of detection for Au absorption is 0.01 ppm.	+	** The limit of detection for Au absorption is theoretically 0.01 ppm, but in actuality is around 2-4 ppm.

	==Purpose of Use==		==Purpose of Use==

2012-12-02T21:51:21Z

Specifications

←Older revision		Revision as of 21:51, 2 December 2012
Line 66:		Line 66:
	* Ambient temperature range: 10~35°C		* Ambient temperature range: 10~35°C
	* Ambient humidity range: 45~80%, but less than 70% if temperature is above 30°C		* Ambient humidity range: 45~80%, but less than 70% if temperature is above 30°C
-	~~<br>~~	+	* Repeatability: Under optimal conditions, RSD 0.5-1.5%
		+	* Accuracy: Affected by sample matrix and method of calibration
		+
	===Calibration Curve of Au Standards===		===Calibration Curve of Au Standards===
	* The AAS was calibrated with solutions of HAuCl<sub>4</sub> in 1 N HCl at the following concentrations: 5 ppm, 8 ppm, 10 ppm, 15 ppm, 20 ppm, 25 ppm, 30 ppm, and 40 ppm.		* The AAS was calibrated with solutions of HAuCl<sub>4</sub> in 1 N HCl at the following concentrations: 5 ppm, 8 ppm, 10 ppm, 15 ppm, 20 ppm, 25 ppm, 30 ppm, and 40 ppm.

2012-12-02T21:39:31Z

Specifications

←Older revision		Revision as of 21:39, 2 December 2012
Line 67:		Line 67:
	* Ambient humidity range: 45~80%, but less than 70% if temperature is above 30°C		* Ambient humidity range: 45~80%, but less than 70% if temperature is above 30°C
	<br>		<br>
-	* Calibration Curve of Au Standards	+	===Calibration Curve of Au Standards===
-	** The AAS was calibrated with solutions of HAuCl<sub>4</sub> in 1 N HCl at the following concentrations: 5 ppm, 8 ppm, 10 ppm, 15 ppm, 20 ppm, 25 ppm, 30 ppm, and 40 ppm.	+	* The AAS was calibrated with solutions of HAuCl<sub>4</sub> in 1 N HCl at the following concentrations: 5 ppm, 8 ppm, 10 ppm, 15 ppm, 20 ppm, 25 ppm, 30 ppm, and 40 ppm.
	[[Image:AA_standard.png]]		[[Image:AA_standard.png]]

-	* Calibration Curve of Cu Standards	+	===Calibration Curve of Cu Standards===
	* The AAS was calibrated with solutions of CuSO<sub>4</sub> in HPLC grade H<sub>2</sub>O.		* The AAS was calibrated with solutions of CuSO<sub>4</sub> in HPLC grade H<sub>2</sub>O.
	[[Image:Cu%2B2_AAS_Calibration_Curve.png]]		[[Image:Cu%2B2_AAS_Calibration_Curve.png]]

2012-12-02T21:38:58Z

Specifications

←Older revision		Revision as of 21:38, 2 December 2012
Line 68:		Line 68:
	<br>		<br>
	* Calibration Curve of Au Standards		* Calibration Curve of Au Standards
-	** The AAS was calibrated with solutions of HAuCl<sub>4</sub> at the following concentrations: 5 ppm, 8 ppm, 10 ppm, 15 ppm, 20 ppm, 25 ppm, 30 ppm, and 40 ppm.	+	** The AAS was calibrated with solutions of HAuCl<sub>4</sub> in 1 N HCl at the following concentrations: 5 ppm, 8 ppm, 10 ppm, 15 ppm, 20 ppm, 25 ppm, 30 ppm, and 40 ppm.
	[[Image:AA_standard.png]]		[[Image:AA_standard.png]]
		+
		+	* Calibration Curve of Cu Standards
		+	* The AAS was calibrated with solutions of CuSO<sub>4</sub> in HPLC grade H<sub>2</sub>O.
		+	[[Image:Cu%2B2_AAS_Calibration_Curve.png]]

	==References==		==References==

2012-12-02T21:36:09Z

Specifications

←Older revision		Revision as of 21:36, 2 December 2012
Line 66:		Line 66:
	* Ambient temperature range: 10~35°C		* Ambient temperature range: 10~35°C
	* Ambient humidity range: 45~80%, but less than 70% if temperature is above 30°C		* Ambient humidity range: 45~80%, but less than 70% if temperature is above 30°C
		+	<br>
		+	* Calibration Curve of Au Standards
		+	** The AAS was calibrated with solutions of HAuCl<sub>4</sub> at the following concentrations: 5 ppm, 8 ppm, 10 ppm, 15 ppm, 20 ppm, 25 ppm, 30 ppm, and 40 ppm.
		+	[[Image:AA_standard.png]]

	==References==		==References==

2012-12-02T21:34:33Z

Use

←Older revision		Revision as of 21:34, 2 December 2012
Line 36:		Line 36:
	*# Extinguish the flame by pushing the red (extinguish) button.		*# Extinguish the flame by pushing the red (extinguish) button.
	*# Click File -> Export Data to save the data as a text file.		*# Click File -> Export Data to save the data as a text file.
		+	* The following protocols utilize the AAS:
		+	** [[User:Moira_M._Esson/Notebook/CHEM-581/2012/10/05\|Analyzing Cu for ion exchange of PVOH films]]
		+	** [[User:Mary_Mendoza/Notebook/CHEM_571_Experimental_Biological_Chemistry_I/2012/10/09\|Analyzing Au in solutions of AuBSA]]

	==Safety==		==Safety==

2012-12-02T21:29:53Z

Specifications

←Older revision		Revision as of 21:29, 2 December 2012
Line 45:		Line 45:

	==Specifications==		==Specifications==
		+	* Physical: W690 x D425 x H370 mm, 38 kg
	* Optics: Double beam, chopper mirror		* Optics: Double beam, chopper mirror
	* Monochromator: Czerny-Turner monochromator, holographic grating (1600 lines/mm)		* Monochromator: Czerny-Turner monochromator, holographic grating (1600 lines/mm)

2012-12-02T19:24:14Z

Description

←Older revision		Revision as of 19:24, 2 December 2012
Line 3:		Line 3:
	* Shimadzu AA-6200 double-beam atomic absorption spectrophotometer.		* Shimadzu AA-6200 double-beam atomic absorption spectrophotometer.
	* It can detect the concentration of Au and Cu even at very low concentrations (ppm).		* It can detect the concentration of Au and Cu even at very low concentrations (ppm).
-	** The limit of detection for Cu absorption is 0.~~0005~~ ppm.	+	** The limit of detection for Cu absorption is 0.003 ppm.
-	** The limit of detection for Au absorption is 9 ppm.	+	** The limit of detection for Au absorption is 0.01 ppm.

	==Purpose of Use==		==Purpose of Use==

2012-12-02T19:23:25Z

Description

←Older revision		Revision as of 19:23, 2 December 2012
Line 3:		Line 3:
	* Shimadzu AA-6200 double-beam atomic absorption spectrophotometer.		* Shimadzu AA-6200 double-beam atomic absorption spectrophotometer.
	* It can detect the concentration of Au and Cu even at very low concentrations (ppm).		* It can detect the concentration of Au and Cu even at very low concentrations (ppm).
		+	** The limit of detection for Cu absorption is 0.0005 ppm.
		+	** The limit of detection for Au absorption is 9 ppm.

	==Purpose of Use==		==Purpose of Use==

2012-11-28T20:35:22Z

Safety

←Older revision		Revision as of 20:35, 28 November 2012
Line 36:		Line 36:

	==Safety==		==Safety==
		+	* Operate the AAS in a properly vented fume hood.
	* Do not look directly at the flame and never leave the flame burning while unattended.		* Do not look directly at the flame and never leave the flame burning while unattended.
	* Do not touch the casing near the flame, as it will become extremely hot when the flame is ignited.		* Do not touch the casing near the flame, as it will become extremely hot when the flame is ignited.