AU Biomaterials Design Lab:Equipment/LS55 PerkinElmer

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==Purpose of use==
==Purpose of use==
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*''How does this instrument work?''
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'''Instrumentation'''
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*''What can this instrument be used for?''
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The LS55 Perkin Elmer Fluorescence Spectrometer, 230V uses a pusled Xenon source to measure fluorescence, phosphorescence, chemi- and bio- luminescence. The FL WinLab™ software has different modes operation like Scan, TimeDrive, and Ratio Data Collection, increasing the versatility of the instrument. The excitation and emission monochromators can be used independently or simultaneously. The instrument also has the ability to prescan the sample to determine the excitation peak and maximum emission.
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Included with the LS55 Perkin Elmer Fluorescence Spectrometer, 230V is a single-cell sample holder that can hold up to a 1cm pathlength cell and has the option of stirring. The automated polarizer has two filter wheels that have both a horizontal and vertical polarizing element. The polarizer is controlled by the software.
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'''Applications'''
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*Fluorescence, phosphorescence and bio- and chemi-luminescence
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*3D excitation/emission scans
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*3D synchronous and kinetic scans
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*Flat samples, like TLC plates and electrophoresis gels, can be analyzed using the plate reader accessory
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*Wavelength kinetics (both single and multiple wavelengths)
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*Intracellular ion analysis
==Location==
==Location==

Revision as of 10:24, 6 December 2012

Contents

Description

Image

  • Perkin Elmer LS 55 Fluorescence Spectrometer
  • This is a 0.1nm resolution, compact spectrophotometer that uses the windows-compatible FL WinLab™ software that can measure fluorescence, phosphorescence and bio- and chemi-luminescence using a pulsed Xenon lamp source.

Purpose of use

Instrumentation The LS55 Perkin Elmer Fluorescence Spectrometer, 230V uses a pusled Xenon source to measure fluorescence, phosphorescence, chemi- and bio- luminescence. The FL WinLab™ software has different modes operation like Scan, TimeDrive, and Ratio Data Collection, increasing the versatility of the instrument. The excitation and emission monochromators can be used independently or simultaneously. The instrument also has the ability to prescan the sample to determine the excitation peak and maximum emission.

Included with the LS55 Perkin Elmer Fluorescence Spectrometer, 230V is a single-cell sample holder that can hold up to a 1cm pathlength cell and has the option of stirring. The automated polarizer has two filter wheels that have both a horizontal and vertical polarizing element. The polarizer is controlled by the software.

Applications

  • Fluorescence, phosphorescence and bio- and chemi-luminescence
  • 3D excitation/emission scans
  • 3D synchronous and kinetic scans
  • Flat samples, like TLC plates and electrophoresis gels, can be analyzed using the plate reader accessory
  • Wavelength kinetics (both single and multiple wavelengths)
  • Intracellular ion analysis

Location

  • Beeghly 207
  • American University
  • Washington, DC
    • access to the laboratory is restricted; see Dr. Miller, Dr. Hartings or Dr. Fox for access.

Contact

Use

  • How does one use this instrument.
  • Include any relevant links to OWW protocols
  • Include any relevant links to the manufacturer's protocols
  • Note to users: you may note any troubleshooting or log your use on the talk page.

Safety

  • Are there any safety considerations for this instrument?

Specifications

Spectral

  • Wavelength range: 190 to 1100nm
  • Spectral bandwidth: 1nm (190 to 1100nm)
  • Wavelength accuracy: ±0.1nm @ 656.1nm D2 and ±0.3nm (190 to 1100nm)
  • Wavelength repeatability: ±0.1nm

Photometric

  • Photometric system: Double Beam
  • Photometric range: Absorbance: -4 to 4 Abs and Transmittance: 0% to 400%
  • Photometric accuracy: ±0.002 Abs (0.5Abs), ±0.004 Abs (1.0Abs), ±0.006 Abs (2.0Abs)
  • Photometric repeatability : less than ±0.001 Abs (0.5Abs), less than ±0.001 Abs (1Abs), less than ±0.003 Abs (2.0Abs)

Baseline

  • Baseline stability: less than 0.0003 Abs/H @700nm (one hour after light source turned ON)
  • Baseline flatness: within ±0.0006 Abs (190 to 1100nm,one hour after light source turned ON)
  • Noise level: within 0.00005 Abs RMS value (@700nm)

Physical

  • Dimensions (W×D×H): 450(W) x 490(D) x 270(H) and Weight is 15kg
  • Memory: USB memory, Saved as text and UVPC file


Calibration

  • Insert a calibration curve here.

References

Useful links


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