AU Biomaterials Design Lab:Equipment/LS55 PerkinElmer

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*Perkin Elmer LS 55 Fluorescence Spectrometer
*Perkin Elmer LS 55 Fluorescence Spectrometer
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*This is a 1nm resolution, compact spectrophotometer that measures absorbance and transmittance in the ultraviolet and visible range of the electromagnetic spectrum.
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*This is a 0.1nm resolution, compact spectrophotometer that measures fluorescence, phosphorescence and bio- and chemi-luminescence using a pulsed Xenon lamp source.
==Purpose of use==
==Purpose of use==

Revision as of 08:45, 6 December 2012

Contents

Description

Image

  • Perkin Elmer LS 55 Fluorescence Spectrometer
  • This is a 0.1nm resolution, compact spectrophotometer that measures fluorescence, phosphorescence and bio- and chemi-luminescence using a pulsed Xenon lamp source.

Purpose of use

  • How does this instrument work?
  • What can this instrument be used for?

Location

  • Beeghly 207
  • American University
  • Washington, DC
    • access to the laboratory is restricted; see Dr. Miller, Dr. Hartings or Dr. Fox for access.

Contact

Use

  • How does one use this instrument.
  • Include any relevant links to OWW protocols
  • Include any relevant links to the manufacturer's protocols
  • Note to users: you may note any troubleshooting or log your use on the talk page.

Safety

  • Are there any safety considerations for this instrument?

Specifications

Spectral

  • Wavelength range: 190 to 1100nm
  • Spectral bandwidth: 1nm (190 to 1100nm)
  • Wavelength accuracy: ±0.1nm @ 656.1nm D2 and ±0.3nm (190 to 1100nm)
  • Wavelength repeatability: ±0.1nm

Photometric

  • Photometric system: Double Beam
  • Photometric range: Absorbance: -4 to 4 Abs and Transmittance: 0% to 400%
  • Photometric accuracy: ±0.002 Abs (0.5Abs), ±0.004 Abs (1.0Abs), ±0.006 Abs (2.0Abs)
  • Photometric repeatability : less than ±0.001 Abs (0.5Abs), less than ±0.001 Abs (1Abs), less than ±0.003 Abs (2.0Abs)

Baseline

  • Baseline stability: less than 0.0003 Abs/H @700nm (one hour after light source turned ON)
  • Baseline flatness: within ±0.0006 Abs (190 to 1100nm,one hour after light source turned ON)
  • Noise level: within 0.00005 Abs RMS value (@700nm)

Physical

  • Dimensions (W×D×H): 450(W) x 490(D) x 270(H) and Weight is 15kg
  • Memory: USB memory, Saved as text and UVPC file


Calibration

  • Insert a calibration curve here.

References

Useful links


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