AU Biomaterials Design Lab:Equipment/FPLC - Revision history

Mary Mendoza: /* Specifications */

2012-12-11T21:08:14Z

Specifications

←Older revision		Revision as of 21:08, 11 December 2012
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	* The system should be flushed with bacteriostatic solution.		* The system should be flushed with bacteriostatic solution.
	* Pressure settings should match the tubing placed on the instrument.		* Pressure settings should match the tubing placed on the instrument.
-
-	~~==Specifications==~~

2012-12-11T21:03:08Z

Use

←Older revision		Revision as of 21:03, 11 December 2012
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	==Use==		==Use==
	1. Open Unicorn		1. Open Unicorn
		+
	2. Go to the System Control Panel and choose Manual		2. Go to the System Control Panel and choose Manual
		+
	3. Select pump option by setting flow, gradient, buffer, pump wash.		3. Select pump option by setting flow, gradient, buffer, pump wash.
		+
	4. Select the flow path by setting the injection valve and pump inlet.		4. Select the flow path by setting the injection valve and pump inlet.
		+
	5. Choose from the Frac option of how the fraction is desired to be distributed.		5. Choose from the Frac option of how the fraction is desired to be distributed.
		+
	6. Press Execute after all desired parameters have been set.		6. Press Execute after all desired parameters have been set.

2012-12-11T21:02:50Z

Use

←Older revision		Revision as of 21:02, 11 December 2012
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	==Use==		==Use==
		+	1. Open Unicorn
		+	2. Go to the System Control Panel and choose Manual
		+	3. Select pump option by setting flow, gradient, buffer, pump wash.
		+	4. Select the flow path by setting the injection valve and pump inlet.
		+	5. Choose from the Frac option of how the fraction is desired to be distributed.
		+	6. Press Execute after all desired parameters have been set.

	==Safety==		==Safety==

2012-12-10T04:58:39Z

Safety

←Older revision		Revision as of 04:58, 10 December 2012
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	==Safety==		==Safety==
		+	* The instrument should be kept indoors and away from dusty atmosphere or close to spraying water.
		+	* Air inlet should NOT be blocked on the front panels or the outlets on the rear of the instrument.
		+	* Do not use for in vitro purposes.
		+	* Modules and components contain high voltage circuits that can give a lethal electric shock.
		+	* UPC-900 and UV-900 monitor uses high intensity UV light. Do not disassemble while the lamp is on.
		+	* Appropriate tubing should be used. Tightening of tubes should be ensured.
		+	* Do not place waste on top of the instrument.
		+	* Wear appropriate laboratory attire when handling equipment.
		+	* The system should be flushed with bacteriostatic solution.
		+	* Pressure settings should match the tubing placed on the instrument.

	==Specifications==		==Specifications==

2012-12-10T04:42:17Z

Use

2012-12-10T04:41:41Z

Contact

←Older revision		Revision as of 04:41, 10 December 2012
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	For further details and use of the instrument please contact the following faculty:		For further details and use of the instrument please contact the following faculty:

-	*[[User:Matt Hartings]]	+	*[[User:Matt Hartings\|Dr. Matt Hartings]]
-	*[[User:Abigail E. Miller]]	+	*[[User:Abigail E. Miller\|Dr. Abigail Miller]]

	==Use==		==Use==

2012-12-10T04:41:08Z

Location

←Older revision		Revision as of 04:41, 10 December 2012
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	*access to the laboratory is restricted; please contact Dr. Miller, Dr. Hartings or Dr. Fox for access.		*access to the laboratory is restricted; please contact Dr. Miller, Dr. Hartings or Dr. Fox for access.
		+
		+	==Contact==
		+	For further details and use of the instrument please contact the following faculty:
		+
		+	*[[User:Matt Hartings]]
		+	*[[User:Abigail E. Miller]]
		+
		+	==Use==

2012-12-10T04:29:05Z

Location

←Older revision		Revision as of 04:29, 10 December 2012
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	==Location==		==Location==
		+	*Beeghly 207
		+
		+	*American University, Washington, DC
		+
		+	*access to the laboratory is restricted; please contact Dr. Miller, Dr. Hartings or Dr. Fox for access.

2012-12-10T04:28:15Z

Purpose of Use

←Older revision		Revision as of 04:28, 10 December 2012
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	* This is used for rapid purification of proteins at low to medium pressures.		* This is used for rapid purification of proteins at low to medium pressures.
	* The system works by passing the sample through different flowpaths with specific affinities for the constituents in the sample.		* The system works by passing the sample through different flowpaths with specific affinities for the constituents in the sample.
		+
		+	==Location==

2012-12-10T04:27:27Z

Description

←Older revision		Revision as of 04:27, 10 December 2012
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	(2) a monitor for pH and conductivity measurement		(2) a monitor for pH and conductivity measurement
		+
		+	==Purpose of Use==
		+	* This is used for rapid purification of proteins at low to medium pressures.
		+	* The system works by passing the sample through different flowpaths with specific affinities for the constituents in the sample.