AU Biomaterials Design Lab:Equipment/Centrifuge - Revision history

Puja Mody: /* Location */

Puja Mody — Wed, 28 Nov 2012 20:33:16 GMT

Location

←Older revision		Revision as of 20:33, 28 November 2012
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	* American University		* American University
	* Washington, DC		* Washington, DC
-	** access to the room is restricted; see Dr. Miller, Dr. Hartings or Dr. Fox for access.	+	** access to the room is restricted; see Dr. Miller, Dr. Hartings, Dr. Costanzi or Dr. Fox for access.

	==Contact==		==Contact==

Puja Mody at 20:10, 28 November 2012

Puja Mody — Wed, 28 Nov 2012 20:10:59 GMT

←Older revision		Revision as of 20:10, 28 November 2012
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	==Purpose of Use==		==Purpose of Use==
-	*Using different rotors allows for a variety of speeds to be used in the centrifuge, depending on the substances that you are trying to separate out, different speeds and different lengths of time may be necessary.	+	*Using different rotors allows for a variety of speeds to be used in the centrifuge, depending on the substances that you are trying to separate out, different speeds and different lengths of time may be necessary. This Centrifuge can be used to separate out solutions and substances based on their individual densities. More dense substances get pushed down while less dense substances are brought to the top.
	* It is first important to note that the rotar is securely attached in the rotor chamber.		* It is first important to note that the rotar is securely attached in the rotor chamber.
	*For the Sh-3000 rotor, the rotor body had four aluminum buckets which hang from the rotor body on support pins.the buckets accept a variety of adapters: glass and plastic tubes or bottles up to 98mm in diameter.		*For the Sh-3000 rotor, the rotor body had four aluminum buckets which hang from the rotor body on support pins.the buckets accept a variety of adapters: glass and plastic tubes or bottles up to 98mm in diameter.
-	* Because the instrument is spinning at high speeds, it is extremely important to balance out the system. When using the rotor buckets or microplate carriers, in the Sh-3000 rotor, the opposing buckets or micro plate carriers need to be balanced to within 10 grams. For the ~~6 plus super speed centrifuge,~~ the balance needs to be within .01grams of each other.	+	* Because the instrument is spinning at high speeds, it is extremely important to balance out the system. When using the rotor buckets or microplate carriers, in the Sh-3000 rotor, the opposing buckets or micro plate carriers need to be balanced to within 10 grams. For the F21S-8x50y rotor the balance needs to be within .01grams of each other.

-	~~Basic Operation~~	+	* A centrifuge can be used for a multitude of purposes. This parti
-	~~#Turn the machine on~~	+
-	#Open the chamber door and install the rotor, if needed, make sure that the rotor cover is installed and place the rotor on the drive spindle and lock it in place turing the rotor locking knob counterclockwise	+
-	~~#close the chamber door~~	+
-	~~#Set the run screen with the appropriate parameters ( including specifications for temperature, time, and rounds per minute (rpm). Be sure to monitor the~~ centrifuge ~~in the beginning to ensure that no unbalance has been detected.~~	+
-	~~# The centrifuge works by spinning the contents of the rotor both horizontally and vertically.~~	+
-		+
-	~~This Centrifuge~~ can be used ~~to separate out solutions and substances based on their individual densities. More dense substances get pushed down while less dense substances are brought to the top~~.	+

	==Location==		==Location==
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	**K factor= the clearing factor for the rotor.		**K factor= the clearing factor for the rotor.
	**Critical speed= speed at which any rotor imbalance will produce a driving frequency equal to the resonant frequency of the rotating system. At this speed, the rotor may produce large amplitude vibrations which can be felt int he instrument frame.		**Critical speed= speed at which any rotor imbalance will produce a driving frequency equal to the resonant frequency of the rotating system. At this speed, the rotor may produce large amplitude vibrations which can be felt int he instrument frame.
		+
		+	Basic Operation
		+	#Turn the machine on
		+	#Open the chamber door and install the rotor, if needed, make sure that the rotor cover is installed and place the rotor on the drive spindle and lock it in place turing the rotor locking knob counterclockwise
		+	#close the chamber door
		+	#Set the run screen with the appropriate parameters ( including specifications for temperature, time, and rounds per minute (rpm). Be sure to monitor the centrifuge in the beginning to ensure that no unbalance has been detected.
		+	# The centrifuge works by spinning the contents of the rotor both horizontally and vertically.

	==Safety==		==Safety==

Puja Mody: /* Use */

Puja Mody — Wed, 28 Nov 2012 19:56:40 GMT

Use

←Older revision		Revision as of 19:56, 28 November 2012
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	[[http://bcmp.med.harvard.edu/sites/bcmp.med.harvard.edu/files/facilities/RC6PLUS%20CENTRIFUGE.pdf\| Centrfuge Manual]]		[[http://bcmp.med.harvard.edu/sites/bcmp.med.harvard.edu/files/facilities/RC6PLUS%20CENTRIFUGE.pdf\| Centrfuge Manual]]
	* Key terms to know		* Key terms to know
-	Relative centrifugal force refers to the force during centrifugation that moves the particulate outward from the center of rotation. force is proportional to the radial distance and the square of the rotor speed.	+	**Relative centrifugal force refers to the force during centrifugation that moves the particulate outward from the center of rotation. force is proportional to the radial distance and the square of the rotor speed.
-	K factor= the clearing factor for the rotor.	+	**K factor= the clearing factor for the rotor.
-	Critical speed= speed at which any rotor imbalance will produce a driving frequency equal to the resonant frequency of the rotating system. At ~~thsi~~ speed, the rotor may produce large amplitude vibrations which can be felt int he instrument frame.	+	**Critical speed= speed at which any rotor imbalance will produce a driving frequency equal to the resonant frequency of the rotating system. At this speed, the rotor may produce large amplitude vibrations which can be felt int he instrument frame.

	==Safety==		==Safety==

Puja Mody: /* Specifications */

Puja Mody — Wed, 28 Nov 2012 19:54:49 GMT

Specifications

←Older revision		Revision as of 19:54, 28 November 2012
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	* THE RC 6-PLUS		* THE RC 6-PLUS
-	Process 4 liters of sample in a single run	+	**Process 4 liters of sample in a single run
-	9 acceleration and 10 deceleration settings	+	**9 acceleration and 10 deceleration settings
-	wide range of rotors are applicable to this centrifuge	+	** wide range of rotors are applicable to this centrifuge
-	Speed selection range= 300-22000rpm +/- 25 rpm	+	**Speed selection range= 300-22000rpm +/- 25 rpm
-	max RCF= 52200 x g	+	**max RCF= 52200 x g
-	Temp selection range and accuracy= -20°C to 40°C +/- 2°C	+	**Temp selection range and accuracy= -20°C to 40°C +/- 2°C
-	Run time selection range= 1 minute- 99 hours and 59 minutes	+	**Run time selection range= 1 minute- 99 hours and 59 minutes
-	ambient temp range= 2°C to 40°C	+	**ambient temp range= 2°C to 40°C
-	mass (weight)= 350 kg	+	**mass (weight)= 350 kg
-	max capacity= 4000mL	+	**max capacity= 4000mL


	* For SH-3000 rotor (w/ buckets)		* For SH-3000 rotor (w/ buckets)
-	The Design Mass for each rotor compartment is 1805 grams at 4700 rpm.	+	**The Design Mass for each rotor compartment is 1805 grams at 4700 rpm.
-	Diameter= 37.1 cm	+	**Diameter= 37.1 cm
-	Mass= 6000 grams	+	**Mass= 6000 grams
-	number of places=4	+	**number of places=4
-	Max Rotor speed= 4700 rpm	+	**Max Rotor speed= 4700 rpm
-	relative centrigual force (RCF) at max speed= 4575	+	**relative centrigual force (RCF) at max speed= 4575
-	K Factor at Max Speed= 8138	+	**K Factor at Max Speed= 8138
-	Max Compartment mass= 1805 grams	+	**Max Compartment mass= 1805 grams
-	Critical Speed= 900rpm	+	**Critical Speed= 900rpm

	*For SH-3000 (w/microplates)		*For SH-3000 (w/microplates)
-	Diameter= 35.9cm	+	**Diameter= 35.9cm
-	mass= 5275 grams	+	**mass= 5275 grams
-	number of places=4	+	**number of places=4
-	max rotor speed= 4700	+	**max rotor speed= 4700
-	RCP at max speed= 3706	+	**RCP at max speed= 3706
-	K factor at max speed=5024	+	**K factor at max speed=5024
-	Max compartment mass= 987g	+	**Max compartment mass= 987g
-	Critical speed= 900 rpm	+	**Critical speed= 900 rpm

	In swinging bucket rotors, the swings clearance of the bucket and contents as they swing from the vertical to horizontal position during centrifugation. proper clearance must be allowed to prevent the tubes from hitting the rotor body and breaking.		In swinging bucket rotors, the swings clearance of the bucket and contents as they swing from the vertical to horizontal position during centrifugation. proper clearance must be allowed to prevent the tubes from hitting the rotor body and breaking.

	* For the F21S-8x50y rotor		* For the F21S-8x50y rotor
-	code =41	+	**code =41
-	max speed= 20000 rpm	+	**max speed= 20000 rpm
-	max RCF= 47.488	+	**max RCF= 47.488
-	K factor= 739	+	**K factor= 739
-	Max compartment mass= 115grams	+	**Max compartment mass= 115grams
-	max radius= 10.6 cm	+	**max radius= 10.6 cm
-	min.radius= 3.3cm	+	**min.radius= 3.3cm

	==References==		==References==
	*[[http://www.tslabor.hu/depo/Centrifuga/Thermo%20Scientific%20Sorvall%20RC%206%20Plus%20superspeed%20centrifug.pdf\| Thermo-scientific centrifuge]]		*[[http://www.tslabor.hu/depo/Centrifuga/Thermo%20Scientific%20Sorvall%20RC%206%20Plus%20superspeed%20centrifug.pdf\| Thermo-scientific centrifuge]]

Puja Mody: /* Specifications */

Puja Mody — Wed, 28 Nov 2012 19:53:26 GMT

Specifications

←Older revision		Revision as of 19:53, 28 November 2012
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	* THE RC 6-PLUS		* THE RC 6-PLUS
-	-Process 4 liters of sample in a single run	+	Process 4 liters of sample in a single run
-	-9 acceleration and 10 deceleration settings	+	9 acceleration and 10 deceleration settings
-	- wide range of rotors are applicable to this centrifuge	+	wide range of rotors are applicable to this centrifuge
-	-Speed selection range= 300-22000rpm +/- 25 rpm	+	Speed selection range= 300-22000rpm +/- 25 rpm
-	-max RCF= 52200 x g	+	max RCF= 52200 x g
-	-Temp selection range and accuracy= -20°C to 40°C +/- 2°C	+	Temp selection range and accuracy= -20°C to 40°C +/- 2°C
-	-Run time selection range= 1 minute- 99 hours and 59 minutes	+	Run time selection range= 1 minute- 99 hours and 59 minutes
-	-ambient temp range= 2°C to 40°C	+	ambient temp range= 2°C to 40°C
-	-mass (weight)= 350 kg	+	mass (weight)= 350 kg
-	-max capacity= 4000mL	+	max capacity= 4000mL


	* For SH-3000 rotor (w/ buckets)		* For SH-3000 rotor (w/ buckets)
-	-The Design Mass for each rotor compartment is 1805 grams at 4700 rpm.	+	The Design Mass for each rotor compartment is 1805 grams at 4700 rpm.
-	-Diameter= 37.1 cm	+	Diameter= 37.1 cm
-	-Mass= 6000 grams	+	Mass= 6000 grams
-	-number of places=4	+	number of places=4
-	- Max Rotor speed= 4700 rpm	+	Max Rotor speed= 4700 rpm
-	-relative centrigual force (RCF) at max speed= 4575	+	relative centrigual force (RCF) at max speed= 4575
-	-K Factor at Max Speed= 8138	+	K Factor at Max Speed= 8138
	Max Compartment mass= 1805 grams		Max Compartment mass= 1805 grams
	Critical Speed= 900rpm		Critical Speed= 900rpm

	*For SH-3000 (w/microplates)		*For SH-3000 (w/microplates)
-	-Diameter= 35.9cm	+	Diameter= 35.9cm
-	-mass= 5275 grams	+	mass= 5275 grams
-	-number of places=4	+	number of places=4
-	-max rotor speed= 4700	+	max rotor speed= 4700
-	-RCP at max speed= 3706	+	RCP at max speed= 3706
-	-K factor at max speed=5024	+	K factor at max speed=5024
-	-Max compartment mass= 987g	+	Max compartment mass= 987g
-	-Critical speed= 900 rpm	+	Critical speed= 900 rpm

	In swinging bucket rotors, the swings clearance of the bucket and contents as they swing from the vertical to horizontal position during centrifugation. proper clearance must be allowed to prevent the tubes from hitting the rotor body and breaking.		In swinging bucket rotors, the swings clearance of the bucket and contents as they swing from the vertical to horizontal position during centrifugation. proper clearance must be allowed to prevent the tubes from hitting the rotor body and breaking.

	* For the F21S-8x50y rotor		* For the F21S-8x50y rotor
-	-code =41	+	code =41
-	-max speed= 20000 rpm	+	max speed= 20000 rpm
-	-max RCF= 47.488	+	max RCF= 47.488
-	-K factor= 739	+	K factor= 739
-	-Max compartment mass= 115grams	+	Max compartment mass= 115grams
-	-max radius= 10.6 cm	+	max radius= 10.6 cm
-	-min.radius= 3.3cm	+	min.radius= 3.3cm
-		+
-		+

	==References==		==References==
	*[[http://www.tslabor.hu/depo/Centrifuga/Thermo%20Scientific%20Sorvall%20RC%206%20Plus%20superspeed%20centrifug.pdf\| Thermo-scientific centrifuge]]		*[[http://www.tslabor.hu/depo/Centrifuga/Thermo%20Scientific%20Sorvall%20RC%206%20Plus%20superspeed%20centrifug.pdf\| Thermo-scientific centrifuge]]

Puja Mody at 19:52, 28 November 2012

Puja Mody — Wed, 28 Nov 2012 19:52:17 GMT

←Older revision		Revision as of 19:52, 28 November 2012
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	* Sorvall F21S-8x50y rotor		* Sorvall F21S-8x50y rotor
	** This instrumentation is used to rotate an object at varying speeds in order to separate out different substances based on their different densities at controlled temperatures. More dense substances are pushed to the bottom while less dense substances are able to move to the top. Between the two rotors, the rpm ranges from 300-22000rpm.		** This instrumentation is used to rotate an object at varying speeds in order to separate out different substances based on their different densities at controlled temperatures. More dense substances are pushed to the bottom while less dense substances are able to move to the top. Between the two rotors, the rpm ranges from 300-22000rpm.
-

	==Purpose of Use==		==Purpose of Use==
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	-number of places=4		-number of places=4
	-max rotor speed= 4700		-max rotor speed= 4700
-	RCP at max speed= 3706	+	-RCP at max speed= 3706
-	K factor at max speed=5024	+	-K factor at max speed=5024
-	Max compartment mass= 987g	+	-Max compartment mass= 987g
-	Critical speed= 900 rpm	+	-Critical speed= 900 rpm

	In swinging bucket rotors, the swings clearance of the bucket and contents as they swing from the vertical to horizontal position during centrifugation. proper clearance must be allowed to prevent the tubes from hitting the rotor body and breaking.		In swinging bucket rotors, the swings clearance of the bucket and contents as they swing from the vertical to horizontal position during centrifugation. proper clearance must be allowed to prevent the tubes from hitting the rotor body and breaking.

	* For the F21S-8x50y rotor		* For the F21S-8x50y rotor
-	-code#=41	+	-code =41
	-max speed= 20000 rpm		-max speed= 20000 rpm
-	-max RCF=47.488	+	-max RCF= 47.488
-	-K factor=739	+	-K factor= 739
	-Max compartment mass= 115grams		-Max compartment mass= 115grams
	-max radius= 10.6 cm		-max radius= 10.6 cm
-	-min. radius= 3.3cm	+	-min.radius= 3.3cm
		+
		+

	==References==		==References==
	*[[http://www.tslabor.hu/depo/Centrifuga/Thermo%20Scientific%20Sorvall%20RC%206%20Plus%20superspeed%20centrifug.pdf\| Thermo-scientific centrifuge]]		*[[http://www.tslabor.hu/depo/Centrifuga/Thermo%20Scientific%20Sorvall%20RC%206%20Plus%20superspeed%20centrifug.pdf\| Thermo-scientific centrifuge]]

Puja Mody at 19:51, 28 November 2012

Puja Mody — Wed, 28 Nov 2012 19:51:06 GMT

←Older revision		Revision as of 19:51, 28 November 2012
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-
	==Description==		==Description==
	Thermo Scientific RC-6+ Centrifuge: two different rotars are available with this model.		Thermo Scientific RC-6+ Centrifuge: two different rotars are available with this model.
Line 34:		Line 33:

	==Use==		==Use==
		+	[[http://bcmp.med.harvard.edu/sites/bcmp.med.harvard.edu/files/facilities/RC6PLUS%20CENTRIFUGE.pdf\| Centrfuge Manual]]
		+	* Key terms to know
	Relative centrifugal force refers to the force during centrifugation that moves the particulate outward from the center of rotation. force is proportional to the radial distance and the square of the rotor speed.		Relative centrifugal force refers to the force during centrifugation that moves the particulate outward from the center of rotation. force is proportional to the radial distance and the square of the rotor speed.
	K factor= the clearing factor for the rotor.		K factor= the clearing factor for the rotor.

Puja Mody at 19:48, 28 November 2012

Puja Mody — Wed, 28 Nov 2012 19:48:54 GMT

←Older revision		Revision as of 19:48, 28 November 2012
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-

	==Description==		==Description==
	Thermo Scientific RC-6+ Centrifuge: two different rotars are available with this model.		Thermo Scientific RC-6+ Centrifuge: two different rotars are available with this model.
	*Sorvall SH-3000 Swinging Bucket Rotor		*Sorvall SH-3000 Swinging Bucket Rotor
-	* Sorvall RC-~~6 Plus Superspeed Centrifuge~~	+	* Sorvall F21S-8x50y rotor
-	** This instrumentation is used to rotate an object at varying speeds in order to separate out different substances based on their different densities at controlled temperatures. More dense substances are pushed to the bottom while less dense substances are able to move to the top. Between the two rotors, the rpm ranges from 0-22000rpm.	+	** This instrumentation is used to rotate an object at varying speeds in order to separate out different substances based on their different densities at controlled temperatures. More dense substances are pushed to the bottom while less dense substances are able to move to the top. Between the two rotors, the rpm ranges from 300-22000rpm.


Line 35:		Line 34:

	==Use==		==Use==
-		+	Relative centrifugal force refers to the force during centrifugation that moves the particulate outward from the center of rotation. force is proportional to the radial distance and the square of the rotor speed.
		+	K factor= the clearing factor for the rotor.
		+	Critical speed= speed at which any rotor imbalance will produce a driving frequency equal to the resonant frequency of the rotating system. At thsi speed, the rotor may produce large amplitude vibrations which can be felt int he instrument frame.

	==Safety==		==Safety==
Line 48:		Line 49:
	==Specifications==		==Specifications==

-	* ~~For the SH~~-~~3000~~	+	* THE RC 6-PLUS
-	~~The Design Mass for each rotor compartment is 1805 grams at 4700~~ rpm.	+	-Process 4 liters of sample in a single run
		+	-9 acceleration and 10 deceleration settings
		+	- wide range of rotors are applicable to this centrifuge
		+	-Speed selection range= 300-22000rpm +/- 25 rpm
		+	-max RCF= 52200 x g
		+	-Temp selection range and accuracy= -20°C to 40°C +/- 2°C
		+	-Run time selection range= 1 minute- 99 hours and 59 minutes
		+	-ambient temp range= 2°C to 40°C
		+	-mass (weight)= 350 kg
		+	-max capacity= 4000mL

-	* For SH-3000 (w/ buckets)	+
		+	* For SH-3000 rotor (w/ buckets)
		+	-The Design Mass for each rotor compartment is 1805 grams at 4700 rpm.
	-Diameter= 37.1 cm		-Diameter= 37.1 cm
	-Mass= 6000 grams		-Mass= 6000 grams
Line 72:		Line 84:

	In swinging bucket rotors, the swings clearance of the bucket and contents as they swing from the vertical to horizontal position during centrifugation. proper clearance must be allowed to prevent the tubes from hitting the rotor body and breaking.		In swinging bucket rotors, the swings clearance of the bucket and contents as they swing from the vertical to horizontal position during centrifugation. proper clearance must be allowed to prevent the tubes from hitting the rotor body and breaking.
		+
		+	* For the F21S-8x50y rotor
		+	-code#=41
		+	-max speed= 20000 rpm
		+	-max RCF=47.488
		+	-K factor=739
		+	-Max compartment mass= 115grams
		+	-max radius= 10.6 cm
		+	-min. radius= 3.3cm

	==References==		==References==
	*[[http://www.tslabor.hu/depo/Centrifuga/Thermo%20Scientific%20Sorvall%20RC%206%20Plus%20superspeed%20centrifug.pdf\| Thermo-scientific centrifuge]]		*[[http://www.tslabor.hu/depo/Centrifuga/Thermo%20Scientific%20Sorvall%20RC%206%20Plus%20superspeed%20centrifug.pdf\| Thermo-scientific centrifuge]]

Puja Mody: /* Safety */

Puja Mody — Wed, 28 Nov 2012 19:19:58 GMT

Safety

←Older revision		Revision as of 19:19, 28 November 2012
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	* Do not operate centrifuge with a rotor out of balance or at the critical speed as this will damage the centrifuge and reduce the component life.		* Do not operate centrifuge with a rotor out of balance or at the critical speed as this will damage the centrifuge and reduce the component life.
	* Make sure centrifuge is grounded properly to avoid electrical shock hazards.		* Make sure centrifuge is grounded properly to avoid electrical shock hazards.
-	*DO not centrifuge materials in this instrument capable of developing flammable or explosive vapors or extreme exothermic reactions.	+	*DO not centrifuge materials in this instrument capable of developing flammable or explosive vapors or extreme exothermic reactions.
		+	* Maintain a clearance around the centrifuge of 30cm or more in all directions.

	==Specifications==		==Specifications==

Puja Mody: /* Specifications */

Puja Mody — Wed, 28 Nov 2012 19:18:28 GMT

Specifications

←Older revision		Revision as of 19:18, 28 November 2012
Line 46:		Line 46:

	==Specifications==		==Specifications==
		+
		+	* For the SH-3000
		+	The Design Mass for each rotor compartment is 1805 grams at 4700 rpm.

		+	* For SH-3000 (w/ buckets)
		+	-Diameter= 37.1 cm
		+	-Mass= 6000 grams
		+	-number of places=4
		+	- Max Rotor speed= 4700 rpm
		+	-relative centrigual force (RCF) at max speed= 4575
		+	-K Factor at Max Speed= 8138
		+	Max Compartment mass= 1805 grams
		+	Critical Speed= 900rpm

		+	*For SH-3000 (w/microplates)
		+	-Diameter= 35.9cm
		+	-mass= 5275 grams
		+	-number of places=4
		+	-max rotor speed= 4700
		+	RCP at max speed= 3706
		+	K factor at max speed=5024
		+	Max compartment mass= 987g
		+	Critical speed= 900 rpm

-		+	In swinging bucket rotors, the swings clearance of the bucket and contents as they swing from the vertical to horizontal position during centrifugation. proper clearance must be allowed to prevent the tubes from hitting the rotor body and breaking.

	==References==		==References==
	*[[http://www.tslabor.hu/depo/Centrifuga/Thermo%20Scientific%20Sorvall%20RC%206%20Plus%20superspeed%20centrifug.pdf\| Thermo-scientific centrifuge]]		*[[http://www.tslabor.hu/depo/Centrifuga/Thermo%20Scientific%20Sorvall%20RC%206%20Plus%20superspeed%20centrifug.pdf\| Thermo-scientific centrifuge]]