ATc

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(Preparing working concentration)
Current revision (21:37, 17 May 2012) (view source)
(Preparing working concentration)
 
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<pre>
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Anhydrotetracycline Prepare a 1,000 times stock solution:   
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Anhydrotetracycline:
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prepare a 100 ug/mL solution in 50% ethanol. Store in a foil-covered tube at -20 °C.
+
Prepare a 1,000 times stock solution:   
 +
100 ug/mL solution in 50% ethanol. Store in a foil-covered tube at -20 °C.
</pre>
</pre>

Current revision

anhydrotetracycline (ATc) is a tetracycline analog, showing increased (30x) affinity for tet repressor. 42 ng/mL gives induction at half maximum according to an in vitro assay.

Preparing working concentration

According to Nallamsetty and Waugh (2007):


Anhydrotetracycline:
Prepare a 1,000 times stock solution:  
100 ug/mL solution in 50% ethanol. Store in a foil-covered tube at -20 °C.

MW = 468.22 g/mole. Thus 1X for the recipe above = 214 nM.

References

Tetracycline analogs affecting binding to Tn10-Encoded Tet repressor trigger the same mechanism of induction.

[1]

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