ATc

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Current revision (20:37, 17 May 2012) (view source)
(Preparing working concentration)
 
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anhydrotetracycline (ATc) is a tetracycline analog, showing increased (30x) affinity for tet repressor. 100 ng/mL gives induction at half maximum.
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anhydrotetracycline (ATc) is a tetracycline analog, showing increased (30x) affinity for tet repressor. 42 ng/mL gives induction at half maximum according to an in vitro assay.
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==Preparing working concentration==
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According to Nallamsetty and Waugh (2007):
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<pre>
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Anhydrotetracycline:
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Prepare a 1,000 times stock solution: 
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100 ug/mL solution in 50% ethanol. Store in a foil-covered tube at -20 °C.
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</pre>
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MW = 468.22 g/mole.  Thus 1X for the recipe above = 214 nM.
==References==
==References==
[http://www.ncbi.nlm.nih.gov/entrez/utils/lofref.fcgi?PrId=3001&uid=8652521&db=pubmed&url=http://dx.doi.org/10.1021/bi952683e Tetracycline analogs affecting binding to Tn10-Encoded Tet repressor trigger the same mechanism of induction.]<br>
[http://www.ncbi.nlm.nih.gov/entrez/utils/lofref.fcgi?PrId=3001&uid=8652521&db=pubmed&url=http://dx.doi.org/10.1021/bi952683e Tetracycline analogs affecting binding to Tn10-Encoded Tet repressor trigger the same mechanism of induction.]<br>
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[http://www.nature.com/nprot/journal/v2/n2/full/nprot.2007.50.html#a1] <br>
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[[Category:Material]] [[Category:Chemical]]

Current revision

anhydrotetracycline (ATc) is a tetracycline analog, showing increased (30x) affinity for tet repressor. 42 ng/mL gives induction at half maximum according to an in vitro assay.

Preparing working concentration

According to Nallamsetty and Waugh (2007):


Anhydrotetracycline:
Prepare a 1,000 times stock solution:  
100 ug/mL solution in 50% ethanol. Store in a foil-covered tube at -20 °C.

MW = 468.22 g/mole. Thus 1X for the recipe above = 214 nM.

References

Tetracycline analogs affecting binding to Tn10-Encoded Tet repressor trigger the same mechanism of induction.

[1]

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