ATc
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Current revision (21:37, 17 May 2012) (view source) (→Preparing working concentration) |
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| - | anhydrotetracycline (ATc) is a tetracycline analog, showing increased (30x) affinity for tet repressor. | + | anhydrotetracycline (ATc) is a tetracycline analog, showing increased (30x) affinity for tet repressor. 42 ng/mL gives induction at half maximum according to an in vitro assay. |
==Preparing working concentration== | ==Preparing working concentration== | ||
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<pre> | <pre> | ||
| - | Anhydrotetracycline Prepare a 1,000 times stock solution | + | Anhydrotetracycline: |
| - | in 50% ethanol | + | Prepare a 1,000 times stock solution: |
| + | 100 ug/mL solution in 50% ethanol. Store in a foil-covered tube at -20 °C. | ||
</pre> | </pre> | ||
| + | |||
| + | MW = 468.22 g/mole. Thus 1X for the recipe above = 214 nM. | ||
==References== | ==References== | ||
Current revision
anhydrotetracycline (ATc) is a tetracycline analog, showing increased (30x) affinity for tet repressor. 42 ng/mL gives induction at half maximum according to an in vitro assay.
Preparing working concentration
According to Nallamsetty and Waugh (2007):
Anhydrotetracycline: Prepare a 1,000 times stock solution: 100 ug/mL solution in 50% ethanol. Store in a foil-covered tube at -20 °C.
MW = 468.22 g/mole. Thus 1X for the recipe above = 214 nM.
