ATc: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
|||
| Line 5: | Line 5: | ||
<pre> | <pre> | ||
Anhydrotetracycline Prepare a 1,000 times stock solution | Anhydrotetracycline Prepare a 1,000 times stock solution: | ||
in 50% ethanol | prepare a 100 ug/mL solution in 50% ethanol. Store in a foil-covered tube at -20 °C. | ||
</pre> | </pre> | ||
MW = 468.22 g/mole. Thus 1X for the recipe above = 214 nM. | MW = 468.22 g/mole. Thus 1X for the recipe above = 214 nM. | ||
==References== | ==References== | ||
Revision as of 18:36, 17 May 2012
anhydrotetracycline (ATc) is a tetracycline analog, showing increased (30x) affinity for tet repressor. 42 ng/mL gives induction at half maximum according to an in vitro assay.
Preparing working concentration
According to Nallamsetty and Waugh (2007):
Anhydrotetracycline Prepare a 1,000 times stock solution: prepare a 100 ug/mL solution in 50% ethanol. Store in a foil-covered tube at -20 °C.
MW = 468.22 g/mole. Thus 1X for the recipe above = 214 nM.
