ATc
From OpenWetWare
(Difference between revisions)
| Line 9: | Line 9: | ||
</pre> | </pre> | ||
| + | |||
| + | MW = 468.22 g/mole. Thus 1X for the recipe above = 214 nM. | ||
==References== | ==References== | ||
Revision as of 18:06, 13 April 2012
anhydrotetracycline (ATc) is a tetracycline analog, showing increased (30x) affinity for tet repressor. 42 ng/mL gives induction at half maximum according to an in vitro assay.
Preparing working concentration
According to Nallamsetty and Waugh (2007):
Anhydrotetracycline Prepare a 1,000 times stock solution by dissolving in 50% ethanol at 100 ug/ml. Store in a foil-covered tube at -20 °C.
MW = 468.22 g/mole. Thus 1X for the recipe above = 214 nM.
