AB medium - Revision history

Nadine Zimmer: /* Recipe */

Nadine Zimmer — Thu, 11 Oct 2012 13:44:41 GMT

Recipe

←Older revision		Revision as of 13:44, 11 October 2012
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	## For CM735 add 20 μg/ml methionine (10 ml of 2mg/ml stock), 20 μg/ml tryptophane (5 ml of 4mg/ml stock) and 25 μg/ml histidine (1 ml of 25 mg/ml stock);		## For CM735 add 20 μg/ml methionine (10 ml of 2mg/ml stock), 20 μg/ml tryptophane (5 ml of 4mg/ml stock) and 25 μg/ml histidine (1 ml of 25 mg/ml stock);
	# 10μg/ml thiamine (1 ml of sterile 10 mg/ml stock)		# 10μg/ml thiamine (1 ml of sterile 10 mg/ml stock)
-	# 25μg/ml uridine (0.25 ml of sterile 10 mg/ml stock)(uridine makes E. coli K12 wildtypes as MG1655 of W3110 grow better because they are starving from pyrimidine <cite>Jensen-JB-1993</cite>.)	+	# 25μg/ml uridine (2.5 ml of sterile 10 mg/ml stock)(uridine makes E. coli K12 wildtypes as MG1655 of W3110 grow better because they are starving from pyrimidine <cite>Jensen-JB-1993</cite>.)

	==References==		==References==

Torsten Waldminghaus — Tue, 03 Jul 2012 12:48:21 GMT

←Older revision		Revision as of 12:48, 3 July 2012
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	*Please feel free to edit this page, add comments on the talk page or contact the page beginner [[User:Torsten Waldminghaus\|Torsten Waldminghaus]].		*Please feel free to edit this page, add comments on the talk page or contact the page beginner [[User:Torsten Waldminghaus\|Torsten Waldminghaus]].

-	[[Category:Material]][[Category:Defined ~~Media]][[Category:~~Media]]	+	[[Category:Material]][[Category:Defined Media]]

Austin J. Che — Wed, 02 Apr 2008 15:46:40 GMT

←Older revision		Revision as of 15:46, 2 April 2008
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	==Purpose==		==Purpose==
-	*AB medium is a minimal growth medium used for bacterial cultures. It has some changes compared to [[M9 medium]] and is supposed to ~~course~~ less problems with precipitation <cite>Clark-JMB-1967</cite>.	+	*AB medium is a minimal growth medium used for bacterial cultures. It has some changes compared to [[M9 medium]] and is supposed to cause less problems with precipitation <cite>Clark-JMB-1967</cite>.

	==Recipe==		==Recipe==

Torsten Waldminghaus — Wed, 02 Apr 2008 14:39:27 GMT

←Older revision		Revision as of 14:39, 2 April 2008
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	#Carbon source (has to be added):		#Carbon source (has to be added):
-	## 0.2% glucose (10ml of 20% or 4ml of 50% sterile stock)	+	## 0.2% [[glucose]] (10ml of 20% or 4ml of 50% sterile stock)
-	## 0.4% acetate (20ml of 20% sterile sodium-acetate stock)	+	## 0.4% acetate (20ml of 20% sterile [[sodium-acetate]] stock)
	#Amino acids (check AA requirement of your [[E. coli genotypes \| strain]]):		#Amino acids (check AA requirement of your [[E. coli genotypes \| strain]]):
	## 0.5% [[casamino acids]] (50ml of 10% sterile stock)		## 0.5% [[casamino acids]] (50ml of 10% sterile stock)

Torsten Waldminghaus — Mon, 31 Mar 2008 09:20:00 GMT

←Older revision		Revision as of 09:20, 31 March 2008
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	Mix 200ml 5xA and 800ml B to get 1 liter AB. Do never add B salts to 5xA!		Mix 200ml 5xA and 800ml B to get 1 liter AB. Do never add B salts to 5xA!
		+
		+	If you want''' agar-plates''' add 16g agar to 1xB, autoclave and than add 5xA.

Torsten Waldminghaus — Mon, 31 Mar 2008 09:16:53 GMT

←Older revision		Revision as of 09:16, 31 March 2008
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	#Amino acids (check AA requirement of your [[E. coli genotypes \| strain]]):		#Amino acids (check AA requirement of your [[E. coli genotypes \| strain]]):
	## 0.5% [[casamino acids]] (50ml of 10% sterile stock)		## 0.5% [[casamino acids]] (50ml of 10% sterile stock)
		+	## For CM735 add 20 μg/ml methionine (10 ml of 2mg/ml stock), 20 μg/ml tryptophane (5 ml of 4mg/ml stock) and 25 μg/ml histidine (1 ml of 25 mg/ml stock);
	# 10μg/ml thiamine (1 ml of sterile 10 mg/ml stock)		# 10μg/ml thiamine (1 ml of sterile 10 mg/ml stock)
	# 25μg/ml uridine (0.25 ml of sterile 10 mg/ml stock)(uridine makes E. coli K12 wildtypes as MG1655 of W3110 grow better because they are starving from pyrimidine <cite>Jensen-JB-1993</cite>.)		# 25μg/ml uridine (0.25 ml of sterile 10 mg/ml stock)(uridine makes E. coli K12 wildtypes as MG1655 of W3110 grow better because they are starving from pyrimidine <cite>Jensen-JB-1993</cite>.)

Torsten Waldminghaus — Thu, 27 Mar 2008 13:38:38 GMT

←Older revision		Revision as of 13:38, 27 March 2008
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	<biblio>		<biblio>
	#Clark-JMB-1967 Clark, DJ and Maaløe, O '''DNA replication and the division cycle in Escherichia coli''' Journal of Molecular Biology		#Clark-JMB-1967 Clark, DJ and Maaløe, O '''DNA replication and the division cycle in Escherichia coli''' Journal of Molecular Biology
-	Volume 23, Issue 1, 14 January 1967, Pages 99-112	+	Volume 23, Issue 1, 14 January 1967, Pages 99-112 [[doi:10.1016/S0022-2836(67)80070-6 \| abstract]]
	#Jensen-JB-1993 pmid=8501045		#Jensen-JB-1993 pmid=8501045
	</biblio>		</biblio>

Torsten Waldminghaus — Thu, 27 Mar 2008 12:37:07 GMT

←Older revision		Revision as of 12:37, 27 March 2008
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	\|}		\|}

-	Mix A and B~~. This makes~~ AB. Do never add B salts to 5xA!	+	Mix 200ml 5xA and 800ml B to get 1 liter AB. Do never add B salts to 5xA!

Torsten Waldminghaus — Thu, 27 Mar 2008 12:34:42 GMT

add supplements

←Older revision		Revision as of 12:34, 27 March 2008
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	==Contact==		==Contact==
-	*Please feel free to edit this page, add comments on the talk page or contact the page beginner ~~'''~~[[User:Torsten Waldminghaus\|Torsten Waldminghaus]] ~~06:12, 27 March 2008 (CDT)'''~~.	+	*Please feel free to edit this page, add comments on the talk page or contact the page beginner [[User:Torsten Waldminghaus\|Torsten Waldminghaus]].

	[[Category:Material]][[Category:Defined Media]][[Category:Media]]		[[Category:Material]][[Category:Defined Media]][[Category:Media]]

Torsten Waldminghaus — Thu, 27 Mar 2008 12:32:59 GMT

add supplements

←Older revision		Revision as of 12:32, 27 March 2008
Line 46:		Line 46:
	#Amino acids (check AA requirement of your [[E. coli genotypes \| strain]]):		#Amino acids (check AA requirement of your [[E. coli genotypes \| strain]]):
	## 0.5% [[casamino acids]] (50ml of 10% sterile stock)		## 0.5% [[casamino acids]] (50ml of 10% sterile stock)
-	# 10μg/ml thiamine (1 ml of sterile 1 mg/ml stock)	+	# 10μg/ml thiamine (1 ml of sterile 10 mg/ml stock)
-		+	# 25μg/ml uridine (0.25 ml of sterile 10 mg/ml stock)(uridine makes E. coli K12 wildtypes as MG1655 of W3110 grow better because they are starving from pyrimidine <cite>Jensen-JB-1993</cite>.)

	==References==		==References==
Line 54:		Line 54:
	#Clark-JMB-1967 Clark, DJ and Maaløe, O '''DNA replication and the division cycle in Escherichia coli''' Journal of Molecular Biology		#Clark-JMB-1967 Clark, DJ and Maaløe, O '''DNA replication and the division cycle in Escherichia coli''' Journal of Molecular Biology
	Volume 23, Issue 1, 14 January 1967, Pages 99-112		Volume 23, Issue 1, 14 January 1967, Pages 99-112
		+	#Jensen-JB-1993 pmid=8501045
	</biblio>		</biblio>