840:153g:Projects/project9/2010/09/30: Difference between revisions

Latest revision as of 13:57, 5 October 2010

We attempted to extract the red florescent biobrick dna containing plasmid by following the bio brick procedure.
The biobrick was not successfully isolated as no bacterial colonies grew.
We prepped our primers for PCR and ran a gradient of temperatures, 44,47.8,51.4,56.2,59.9,64 in the PCR machine to discover our ideal annealing temperature.
Primers were left to run the PCR cycle and then put in the -20 freezer.

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 *We attempted to extract the red florescent biobrick dna containing plasmid by following the bio brick procedure.
-*After following procedure, which added TE buffer and competent cells, the solution was placed in the shaker incubator to be plated by another group.
+*The biobrick was not successfully isolated as no bacterial colonies grew.
-*The biobrick was not successfully isolated.
+*We prepped our primers for PCR and ran a gradient of temperatures, 44,47.8,51.4,56.2,59.9,64 in the PCR machine to discover our ideal annealing temperature.
-*We prepped our primers for PCR and ran a gradient of temperatures in the PCR machine to discover our ideal annealing temperature.
+*Primers were left to run the PCR cycle and then put in the -20 freezer.
-*Primers were left to run the PCR cycle and then put in the -20 freezer for further assessment next week.