840:153g:Projects/project9/2010/09/30: Difference between revisions
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*We attempted to extract the red florescent biobrick dna containing plasmid by following the bio brick procedure. | |||
*After following procedure, which added TE buffer and competent cells, the solution was placed in the shaker incubator to be plated by another group. | |||
*The biobrick was not successfully isolated. | |||
*We prepped our primers for PCR and ran a gradient of temperatures in the PCR machine to discover our ideal annealing temperature. | |||
*Primers were left to run the PCR cycle and then put in the -20 freezer for further assessment next week. |
Revision as of 07:35, 4 October 2010
- We attempted to extract the red florescent biobrick dna containing plasmid by following the bio brick procedure.
- After following procedure, which added TE buffer and competent cells, the solution was placed in the shaker incubator to be plated by another group.
- The biobrick was not successfully isolated.
- We prepped our primers for PCR and ran a gradient of temperatures in the PCR machine to discover our ideal annealing temperature.
- Primers were left to run the PCR cycle and then put in the -20 freezer for further assessment next week.