840:153g:Projects/project4/2009/04/15: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> MoldBusters' Journal</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
==Wednesday 4/15==
* Insert content here...
''Josh''
* The controls for the transformation showed that the transformation worked properly, and that ampicillin resistance was being selected for.
* For the ligation controls, only two colonies grew which contained only the plasmid.
* The most efficient ligation was the 1:1 ratio, and plated in an 8μL quantity. This plate showed 50 colonies. Other colonies were observed on the 2:1 ratio.
* Eleven colonies from the 1:1 ligation and one from the 2:1 ligation were used to inoculate liquid cultures containing ampicillin. Incubated at 37°C , to be used tomorrow for plasmid extraction.




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Revision as of 10:03, 16 April 2009

MoldBusters' Journal <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Wednesday 4/15

Josh

  • The controls for the transformation showed that the transformation worked properly, and that ampicillin resistance was being selected for.
  • For the ligation controls, only two colonies grew which contained only the plasmid.
  • The most efficient ligation was the 1:1 ratio, and plated in an 8μL quantity. This plate showed 50 colonies. Other colonies were observed on the 2:1 ratio.
  • Eleven colonies from the 1:1 ligation and one from the 2:1 ligation were used to inoculate liquid cultures containing ampicillin. Incubated at 37°C , to be used tomorrow for plasmid extraction.


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