840:153g:Projects/project4/2009/04/14: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2009/04/14 Entry for 840:153g:Projects/project4)
 
No edit summary
Line 1: Line 1:
{|{{table}} width="800"
{|{{table}} width="800"
|-
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> MoldBusters' Journal</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
|-
| colspan="2"|
| colspan="2"|
==Entry title==
==Tuesday 4/14==
* Insert content here...
''Josh, Casy, and Oggie''
* The ligation products were used to transform competent cells. The transformation was done with volumes of 2μL and 8μL of DNA from the ligation.
* The transformed cells were spread plated onto LB+Amp plates, and incubated at 37°C.
* On Wednesday, the transformed colonies will be inoculated into liquid cultures containing ampicillin, to be used in plasmid extraction on Thursday.
* A 10% Arabinose solution was made and sterilized for future use in testing expression.




<!-- ## Do not edit below this line unless you know what you are doing. ## -->
<!-- ## Do not edit below this line unless you know what you are doing. ## -->
|}
|}

Revision as of 15:09, 14 April 2009

MoldBusters' Journal <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Tuesday 4/14

Josh, Casy, and Oggie

  • The ligation products were used to transform competent cells. The transformation was done with volumes of 2μL and 8μL of DNA from the ligation.
  • The transformed cells were spread plated onto LB+Amp plates, and incubated at 37°C.
  • On Wednesday, the transformed colonies will be inoculated into liquid cultures containing ampicillin, to be used in plasmid extraction on Thursday.
  • A 10% Arabinose solution was made and sterilized for future use in testing expression.


Retrieved from "https://openwetware.org/mediawiki/index.php?title=840:153g:Projects/project4/2009/04/14&oldid=301798"