840:153g:Projects/project4/2009/04/09: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 11: Line 11:
* The ligation contained the appropriate amount of plasmid and gene, as well as ligase and buffer. Controls to test self-ligation will be done Friday.
* The ligation contained the appropriate amount of plasmid and gene, as well as ligase and buffer. Controls to test self-ligation will be done Friday.
* 800 mL of LB+Amp plates were prepared.
* 800 mL of LB+Amp plates were prepared.
''Oggie and Katy''
* Setup a PCR using old DNA strains from ''B. subtilis'' globiformis and niger in combination with our newly synthesizes aprE primers, and used original aprE primers with the DNA from ''B. subtilis'' strain 168
* Results will hopefully reveal whether our initial failures were due to poorly synthesized primers, different DNA between the strains, or possibly both
* Also setup a PCR to mass produce the wintergreene gene that Katy and Derek are working on




<!-- ## Do not edit below this line unless you know what you are doing. ## -->
<!-- ## Do not edit below this line unless you know what you are doing. ## -->
|}
|}

Revision as of 09:51, 14 April 2009

MoldBusters' Journal <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Thursday 4/9

Josh and Casy

  • Ran a gel of the extracted aprE gene from Tuesday in order to confirm purity and determine concentration. The second elution samples contained little to no DNA. The first elution samples, however, contained a very high concentration of DNA, about 13 ng/μL.
  • Using this concentration of gene and the contration of the digested plasmid, the ligation was setup in ratios of 1:1, 2:1, and 1:5 (Based on molar ratios of plasmid to gene).
  • The ligation contained the appropriate amount of plasmid and gene, as well as ligase and buffer. Controls to test self-ligation will be done Friday.
  • 800 mL of LB+Amp plates were prepared.

Oggie and Katy

  • Setup a PCR using old DNA strains from B. subtilis globiformis and niger in combination with our newly synthesizes aprE primers, and used original aprE primers with the DNA from B. subtilis strain 168
  • Results will hopefully reveal whether our initial failures were due to poorly synthesized primers, different DNA between the strains, or possibly both
  • Also setup a PCR to mass produce the wintergreene gene that Katy and Derek are working on


Retrieved from "https://openwetware.org/mediawiki/index.php?title=840:153g:Projects/project4/2009/04/09&oldid=301749"