840:153g:Projects/project4/2009/03/26: Difference between revisions
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* The PCR will be run with 1μL and 0.1μL of DNA. The temperatures that will range from 45°C, 50°C, 55°C, and 60°C. | * The PCR will be run with 1μL and 0.1μL of DNA. The temperatures that will range from 45°C, 50°C, 55°C, and 60°C. | ||
* The new primers contain a point mutation that will eliminate hairpin formation. | * The new primers contain a point mutation that will eliminate hairpin formation. | ||
Forward Primer: | |||
5’ TAGAATTCGCGGCCGCTTCTAG ATGAG(G)AGCAAAAAATTGTGG | |||
Reverse Primer: | |||
5’ ATCTGCAGCGGCCGCTACTAGTA TTATTGTGCAGC(A)GCTTGTAC | |||
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Revision as of 13:31, 26 March 2009
MoldBusters' Journal | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Thursday 3/26Oggie
Josh and Casy
Forward Primer: 5’ TAGAATTCGCGGCCGCTTCTAG ATGAG(G)AGCAAAAAATTGTGG Reverse Primer: 5’ ATCTGCAGCGGCCGCTACTAGTA TTATTGTGCAGC(A)GCTTGTAC
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