840:153g:Projects/project4/2009/02/26: Difference between revisions

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==Thursday 2/26==
==Thursday 2/26==
''Josh''
''Josh''
* The QAIquick protocol was followed in order to extract the digested plasmid from the agarose gel. Two samples of 47μL dissolved DNA were stored at -20°C.
* The QAIquick protocol was followed in order to extract the digested plasmid from the agarose gel.
* A small sample from each sample (3μL) were run on a gel. It was determined that...
* A small amount from each sample (3μL) was run on a gel. It was determined that Sample 1 was purified, as only a single band at 2000bp was present. Sample 2 had a band at 2000bp, and a smaller band at 1500bp, which corresponded to the undigested plasmid.
* The concentration of the samples are about 0.8ng/μL. Two 27μL samples are stored at -20°C.


''Oggie''
''Oggie''
* Prepared agarose gel and loaded PCR products from Tuesday 2/24
* Prepared agarose gel and loaded PCR products from Tuesday 2/24.
* Analyzed gel and determined that our PCR was once again unsuccessful
* Analyzed gel and determined that our PCR was once again unsuccessful.




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Revision as of 16:34, 26 February 2009

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Thursday 2/26

Josh

  • The QAIquick protocol was followed in order to extract the digested plasmid from the agarose gel.
  • A small amount from each sample (3μL) was run on a gel. It was determined that Sample 1 was purified, as only a single band at 2000bp was present. Sample 2 had a band at 2000bp, and a smaller band at 1500bp, which corresponded to the undigested plasmid.
  • The concentration of the samples are about 0.8ng/μL. Two 27μL samples are stored at -20°C.

Oggie

  • Prepared agarose gel and loaded PCR products from Tuesday 2/24.
  • Analyzed gel and determined that our PCR was once again unsuccessful.


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