840:153g:Projects/project4/2009/02/17: Difference between revisions

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''Oggie and Katy''
''Oggie and Katy''
* Did research on possibility of genetic differences between globiformis & niger strains, and strain 168 (we used DNA from [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=Nucleotide&dopt=GenBank&val=32468723 strain 168] as the template when developing our primers)
* Did research on possibility of genetic differences between globiformis & niger strains, and strain 168 (we used DNA from [http://www.ncbi.nlm.nih.gov/entrez/viewer.fcgi?db=Nucleotide&dopt=GenBank&val=32468723 strain 168] as the template when developing our primers)
* Did not find any results in regards to the two strains we are using
* Found new DNA extraction protocol at [http://www.bio.net/bionet/mm/methods/2003-July/096531.html Bio.net]
* Found new DNA extraction protocol at [http://www.bio.net/bionet/mm/methods/2003-July/096531.html Bio.net]
* Made TES buffer needed for new DNA extraction method
* Made TES buffer needed for new DNA extraction method
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Revision as of 17:13, 18 February 2009

MoldBusters' Journal <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
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Tuesday 2/17

Josh and Casy

  • The plasmid pSB1A2+R0080 samples were combined and digested with Pst1 and Spe1.
  • The samples were purified by running the DNA through a column.
  • Verification that the digestion was a success will be done on Thursday by running a gel.
  • The digested plasmid was stored in the -20°C freezer, and should be ready for the aprE ligation.

Oggie and Katy

  • Did research on possibility of genetic differences between globiformis & niger strains, and strain 168 (we used DNA from strain 168 as the template when developing our primers)
  • Did not find any results in regards to the two strains we are using
  • Found new DNA extraction protocol at Bio.net
  • Made TES buffer needed for new DNA extraction method
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