840:153g:Projects/project4/2009/02/12: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> MoldBusters' Journal</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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== | ==Thursday 2/12== | ||
''Josh and Casy'' | ''Josh and Casy'' | ||
*Came in early to start Agarose gel. Followed protocol discussed Tuesday. | * Came in early to start Agarose gel. Followed protocol discussed Tuesday. | ||
*Prepared the part pSB1A2 + BBa_R0080 as digested DNA and undigested DNA for running on the electrophoresis gel. Digested DNA with the enzymes Xba1 and Spe1. | * Prepared the part pSB1A2 + BBa_R0080 as digested DNA and undigested DNA for running on the electrophoresis gel. Digested DNA with the enzymes Xba1 and Spe1. | ||
*Ran the gel for | * Ran the gel for 30 minutes. | ||
* The gel showed that the digested samples formed two fragments, the smaller of which was about 200 base pairs. This confirmed that the desired part has been isolated. | |||
* Discussed preparing the plasmid for the incorporation of the aprE gene. | |||
Oggie, Derek, and Katy | ''Oggie, Derek, and Katy'' | ||
*Oggie and Derek came in early to make Agarose gel. | * Oggie and Derek came in early to make Agarose gel. | ||
*We checked PCR product using gel electrophoresis. | * We checked PCR product using gel electrophoresis. | ||
*Our + control worked but our DNA did not work. | * Our + control worked but our DNA did not work. | ||
*On Tuesday we will be calibrating pipets and redoing our DNA extraction. | * On Tuesday we will be calibrating pipets and redoing our DNA extraction. | ||
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http://openwetware.org/index.php?title=840:153g:Projects/project4/2009/02/12&action=edit§ion=1 | http://openwetware.org/index.php?title=840:153g:Projects/project4/2009/02/12&action=edit§ion=1 |
Revision as of 19:21, 12 February 2009
MoldBusters' Journal | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Thursday 2/12Josh and Casy
Oggie, Derek, and Katy
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http://openwetware.org/index.php?title=840:153g:Projects/project4/2009/02/12&action=edit§ion=1