840:153g:Projects/project3/2009/04/16: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Tear Inducing Bacteria</span> | ||
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | |style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html> </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}} | ||
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== | ==April 16, 2009== | ||
* | * We ran a gel to confirm PCR results. We lost our P2-R2 RT-PCR step sample somewhere in the freezer and we unable to find it so we only had P1-R2 RT-PCR sample. Our gel showed that there was something in all of them but not of the desired length of 1600-1700 basepairs. It the sample P1-R2 RT-PCR step we could see one light band around 1000 basepairs and a darker band around 400 basepairs. In HOPS RT-PCR we can see a band between 200 and 400 basepairs. This tells us something is happening but we're not quite gettin the desired results. Our positive control worked so we know PCR worked. For Tuesday we will attempt this again for the last time. We are changing the annealing time to 2 minutes and using two temperatures (46, 50 degrees Celsius). We will not be using out positive control. | ||
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Revision as of 13:43, 21 April 2009
 Tear Inducing Bacteria
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<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
April 16, 2009
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